Abstract Traditional leafy vegetables are increasingly being recognized as parents in plant breeding, and source of improved nutrition and food supply. Wild cucumber (Cucumis myriocarpus), a traditional leafy vegetable has been reported to possess some resistance to root-knot (Meloidogyne species) nematodes; however, the form of nematode resistance in these plants is not documented. The objective of this study was to determine the possible mechanism of nematode resistance in C. myriocarpus to M. incognita and M. javanica under greenhouse conditions. Two separate experiments of C. myriocarpus were each inoculated with second-stage juveniles (J2) of M. incognita or M. javanica. Harvesting of seedlings was conducted every other day from 2 days after inoculation. At each harvest, roots of five seedlings were stained using acid fuchsin method for assessment and number of necrotic spots, giant cells, rootlet interference, root galls and nematode juveniles counted. Harvest period had highly significant effects (P ≤ 0.01) on numbers of giant cells, rootlet interference and root galls, but were not significant on necrotic spot number in C. myriocarpus - M. javanica relations. Harvest interval were significant (P ≤ 0.05) on giant cell number and highly significant on root gall number in C. myriocarpus - M. incognita relations. The assessment of nematode infection showed that there was delayed penetration of both M. incognita and M. javanica in C. myriocarpus but their ability to cause disease was impaired. In conclusion, post-infectional resistance is the possible mechanism in C. myriocarpus traditional leafy vegetable against the two Meloidogyne species.

中文翻译：

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根结线虫感染的传统叶类蔬菜的感染后抗性

摘要 传统叶类蔬菜越来越被认为是植物育种的亲本，以及改善营养和食物供应的来源。据报道，野生黄瓜 (Cucumis myriocarpus) 是一种传统的多叶蔬菜，对根结线虫（根结线虫）具有一定的抵抗力；然而，这些植物中线虫抗性的形式没有记录。本研究的目的是确定在温室条件下 C. myriocarpus 对 M. incognita 和 M. javanica 的线虫抗性的可能机制。C. myriocarpus 的两个独立实验分别用 M. incognita 或 M. javanica 的第二阶段幼体 (J2) 接种。从接种后2天起每隔一天进行幼苗的收获。每一次收获，用酸性品红法对5株幼苗根部进行染色，计算坏死斑数、巨细胞数、小根干扰数、根瘿数和线虫幼虫数。收获期对巨细胞数、根干扰和根瘿数有极显着影响（P ≤ 0.01），但对C. myriocarpus - M. javanica关系中的坏死点数不显着。收获间隔对巨果细胞数显着（P ≤ 0.05），对C. myriocarpus - M. incognita 关系中的根瘿数具有高度显着性。线虫感染的评估表明，南方根结线虫和爪哇根结线虫在 C. myriocarpus 中的渗透延迟，但它们引起疾病的能力受损。总之，感染后耐药是 C.