Truffle species indigenous to Japan include a white-colored truffle Tuber japonicum and two black-colored truffles, T. himalayense and T. longispinosum. As the fruiting bodies of these Tuber species are promising edible, studies on the artificial cultivation for these truffles are currently underway in Japan. In the present study, we investigated the influence of pH on in vitro mycelial growth in these Tuber species to determine the optimal pH conditions for the cultivation of these truffles. Mycelia of five strains from each species were cultured in modified Melin–Norkrans liquid medium at different pH values. Tuber japonicum grew well at pH 5.0 and 6.0, whereas T. himalayense and T. longispinosum grew well at pH 7.0. This results suggest that the optimal pH for mycelial growth varies among Tuber species. The growth data collected in this study can be used to design optimal pH conditions for artificial cultivation of these Japanese truffles.

原产于日本松露品种包括白松露色的块茎血吸虫和两个黑色松露，牛逼。himalayense和Ť。长针刺。由于这些块茎种的子实体可以食用，因此日本目前正在对这些松露的人工栽培进行研究。在本研究中，我们调查了pH对这些块茎种体外菌丝生长的影响，以确定用于培养这些松露菌的最佳pH条件。在改良的Melin-Norkrans液体培养基中，在不同的pH值下培养每种菌种的5个菌株的菌丝体。日本块茎在pH 5.0和6.0中生长良好，而Ť。himalayense和Ť。在pH 7.0下，长叶长耳松生长良好。该结果表明，菌丝生长的最佳pH在块茎菌种之间有所不同。在这项研究中收集的生长数据可用于设计这些松露的人工栽培的最佳pH条件。