Biodiesel (fatty acids methyl esters, FAME) has attracted considerable attention as an environmentally and eco-friendly alternative for diesel engines. This research manipulates the use of Aspergillus whole-cell lipase as a biocatalyst for biodiesel production from waste frying oil (WFO). A total of 17 isolates of Aspergillus species screened for lipase and esterase production abilities. Qualitatively, 11 Aspergillus isolates showed lipase and/or esterase activities and only 4 isolates were able to perform WFO transesterification under the tested conditions. Two Aspergillus isolates showed relatively high FAME yields, thus were selected as good enzyme producers. These two isolates were molecularly identified using rRNA gene sequence ITS1 and ITS2 as A. tamarii NDA03a (Genbank Accession Number MK849615) and A. flavus NDA04a (Genbank Accession Number MK811208), respectively. These identified isolates were exposed to ethyl methanesulfonate (EMS) for producing hyper lipolysis mutants. Mutagenesis led to 13.15 and 14.45% improvement of the WFO transesterification by A. tamarii NDA03a and A. flavus NDA04a, respectively. Random amplified polymorphic DNA (RAPD) analysis of the produced mutants confirmed the genetic basis of the activity variation. Genetic polymorphism reached to 79.31% and 80.65% between A. flavus NDA04a and A. tamarii NDA03a mutants and their corresponding wild types, respectively.

生物柴油（脂肪酸甲酯，FAME）作为柴油机的环保和环保替代品已引起了广泛关注。这项研究操纵了曲霉全细胞脂肪酶作为从废油炸油（WFO）生产生物柴油的生物催化剂的用途。总共筛选了17种曲霉菌菌株的脂肪酶和酯酶生产能力。定性地，11株曲霉菌显示脂酶和/或酯酶活性，只有4株在测试条件下能够进行WFO酯交换反应。两个曲霉分离株显示出较高的FAME产量，因此被选为良好的酶生产商。使用rRNA基因序列ITS1和ITS2因为这两个分离物分子鉴定溜曲霉NDA03a（Genbank登录号MK849615）和黄曲霉NDA04a（Genbank登录号MK811208），分别。将这些鉴定出的分离物暴露于甲磺酸乙酯（EMS），以产生高脂解突变体。突变导致了WFO酯交换的13.15和14.45％的改善溜A. NDA03a和黄曲霉NDA04a，分别。产生的突变体的随机扩增多态性DNA（RAPD）分析证实了活性变异的遗传基础。遗传多态性分别达到79.31％和80.65％黄曲霉NDA04a和tamarii NDA03a突变体及其相应的野生型。