SummaryIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production.

中文翻译：

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是否有可能通过减少胎牛血清和使用左旋肉碱来改变胚胎脂质积累来使蟾蜍灵卵母细胞体外成熟？

概括体外胚胎生产（IVEP）是一种可以在短时间内促进遗传改良的程序。然而，这种生物技术在水牛身上获得的成功率仍然不一致，这可能与卵母细胞和胚胎细胞质中脂质的高浓度有关。本研究的目的是评估降低胎牛血清 (FBS) 浓度和/或使用l-肉碱期间体外成熟（IVM）对布巴林胚胎植入前发育和脂质积累的影响。在第一个实验中，确定了 IVM 培养基中 FBS 的最低浓度（0%、2.5%、5% 或 10%），维持良好胚胎发育率的最低浓度为 5%。在第二个实验中，添加 5 mMl对进入成熟培养基的肉碱进行了评估。对产生的囊胚进行脂质评估，包括染色，然后使用光学（油红 O）和共聚焦（BODIPY 493/503）显微镜观察。5% 和 10% FBS 组之间没有观察到差异，优于 0% 和 2.5% 组。此外，用 5% 和 10% FBS 治疗的组的表现优于补充l-肉碱。胚胎脂质积累没有差异。结果表明，可以将 FBS 浓度降低到 5%体外用于生产布巴林胚胎的成熟培养基，并添加 5 mMl-肉碱不会增加胚胎产量。