In this study, antifouling and antibacterial properties of polar, semi polar and non-polar bioactive compounds derived from the sea cucumber Holothuria leucospilota were investigated. A series comprising n-hexane, ethyl acetate and methanol extracts from four different organs of sea cucumber (body wall, gonad, digestive tract and respiratory tree) were investigated for their antibacterial and antifouling activity against two species of microalgae, larvae of barnacle Amphibalanus amphitrite and a non-target organism: larvae of Artemia salina. Results showed that the highest antibacterial activity was found in the ethyl acetate extract of body wall against Staphylococcus aureus with minimum inhibitory concentration (MIC) of 0.250 mg/mL. The MIC of ethyl acetate extract of body wall ranged between 0.062 and 0.250 mg/mL against two microalgal strains. All extracts showed moderate to low toxicity against larvae of barnacles. The most pronounced anti-barnacle activity was found in the ethyl acetate extract of body wall with median lethal concentration (LC50) value of 0.049 mg/mL. Among the twelve extracts, the ethyl acetate extracts of body wall showed the maximum antibacterial and antifouling activities. The bioactive compounds of the ethyl acetate extract of the sea cucumber body wall were determined using gas chromatography coupled with mass spectrometry. Fatty acids and terpenes were the major compounds, which may be responsible for antibacterial and antifouling activity of the ethyl acetate extract of body wall of the sea cucumber H. leucospilota.

中文翻译：

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防污从海参的不同器官的生物活性提取物的抗菌活性玉足海参

在这项研究中，研究了源自海参Holothuria leucospilota的极性，半极性和非极性生物活性化合物的防污和抗菌性能。研究了一系列从海参四个不同器官（体壁，性腺，消化道和呼吸道树）中提取的正己烷，乙酸乙酯和甲醇提取物对两种微藻，藤壶两栖闪石幼虫的抗菌和防污活性。非目标生物：卤虫卤虫的幼虫。结果表明，在体壁乙酸乙酯提取物中对金黄色葡萄球菌具有最高的抗菌活性，最低抑菌浓度（MIC）为0.250 mg / mL。两种微藻菌株的体壁乙酸乙酯提取物的MIC在0.062至0.250 mg / mL之间。所有提取物对藤壶的幼虫均显示中度至低毒。在体壁的乙酸乙酯提取物中发现最明显的抗藤壶活性，其致命中值（LC50）值为0.049 mg / mL。在十二种提取物中，体壁的乙酸乙酯提取物表现出最大的抗菌和防污活性。使用气相色谱-质谱联用技术测定海参体壁乙酸乙酯提取物的生物活性化合物。脂肪酸和萜烯是主要化合物，它们可能对海参隐杆线虫体壁乙酸乙酯提取物的抗菌和防污活性负责。在体壁的乙酸乙酯提取物中发现最明显的抗藤壶活性，其致命中值（LC50）值为0.049 mg / mL。在十二种提取物中，体壁的乙酸乙酯提取物表现出最大的抗菌和防污活性。使用气相色谱-质谱联用技术测定海参体壁乙酸乙酯提取物的生物活性化合物。脂肪酸和萜烯是主要化合物，它们可能负责海参隐杆线虫体壁乙酸乙酯提取物的抗菌和防污活性。在体壁的乙酸乙酯提取物中发现最明显的抗藤壶活性，其致命中值（LC50）值为0.049 mg / mL。在十二种提取物中，体壁的乙酸乙酯提取物表现出最大的抗菌和防污活性。使用气相色谱-质谱联用技术测定海参体壁乙酸乙酯提取物的生物活性化合物。脂肪酸和萜烯是主要化合物，它们可能对海参隐杆线虫体壁乙酸乙酯提取物的抗菌和防污活性负责。使用气相色谱-质谱联用法测定海参体壁乙酸乙酯提取物的生物活性化合物。脂肪酸和萜烯是主要化合物，它们可能对海参隐杆线虫体壁乙酸乙酯提取物的抗菌和防污活性负责。使用气相色谱-质谱联用法测定海参体壁乙酸乙酯提取物的生物活性化合物。脂肪酸和萜烯是主要化合物，它们可能对海参隐杆线虫体壁乙酸乙酯提取物的抗菌和防污活性负责。