Verminephrobacter is a genus of symbiotic bacteria that live in the nephridia of earthworms. The bacteria are recruited during the embryonic stage of the worm and transferred from generation to generation in the same manner. The worm provides shelter and food for the bacteria. The bacteria deliver micronutrients to the worm. The present study reports the genome sequence assembly and annotation of a new strain of Verminephrobacter called Verminephrobacter eiseniae msu. We separated the sequences of a new Verminephrobacter strain from the whole genome of Eisenia fetida using the sequence of V. eiseniae EF01-2, and the bacterial genome was assembled using the CLC Workbench. The de novo-assembled genome was annotated and analyzed for the protein domains, functions, and metabolic pathways. Besides, the multigenome comparison was performed to interpret the phylogenomic relationship of the strain with other proteobacteria. The FastqSifter sifted a total of 593,130 Verminephrobacter genomic reads. The de novo assembly of the reads generated 1832 contigs with a total genome size of 4.4 Mb. The Average Nucleotide Identity denoted the bacterium belongs to the species V. eiseniae, and the 16S rRNA analysis confirmed it as a new strain of V. eiseniae. The AUGUSTUS genome annotation predicted a total of 3809 protein-coding genes; of them, 3805 genes were identified from the homology search. The bioinformatics analysis confirmed the bacterium is an isolate of V. eiseniae, and it was named Verminephrobacter eiseniae msu. The whole genome of the bacteria can be utilized as a useful resource to explore the area of symbiosis further.

中文翻译：

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一种新的艾美金边杆菌菌株的基因组草案：earth的一种肾病共生体

Verminephrobacter是生活在of肾中的一种共生细菌。细菌是在蠕虫的胚胎阶段募集的，并以相同的方式代代相传。蠕虫为细菌提供了庇护所和食物。细菌向蠕虫传递微量营养素。本研究报告了一种名为Verminephrobacter eiseniae msu的新的Verminephrobacter菌株的基因组序列装配和注释。我们使用艾美尼亚弧菌EF01-2的序列，从费氏艾美氏杆菌的整个基因组中分离出一种新的Verminephrobacter菌株的序列，并使用CLC Workbench组装了细菌基因组。注释从头组装的基因组，并分析其蛋白质结构域，功能和代谢途径。除了，进行多基因组比较以解释该菌株与其他蛋白菌的种系关系。FastqSifter共筛查了593,130个红细菌基因组读物。读取的从头组装产生1832个重叠群，总基因组大小为4.4 Mb。平均核苷酸同一性表示该细菌属于艾美尼亚弧菌，并且16S rRNA分析证实该细菌为艾美尼亚弧菌的新菌株。AUGUSTUS基因组注释预测总共有3809个蛋白质编码基因。其中，从同源性搜索中鉴定出3805个基因。生物信息学分析证实该细菌为艾氏弧菌的分离株，并被命名为艾氏Verminephrobacter mise。细菌的整个基因组可用作进一步探索共生领域的有用资源。