The osteoblast‐function associated integrin–ligand interactions are known to be dependent on divalent cations, but there is scarce information about the effect of Ca²⁺ and Mg²⁺ adsorption properties of an implant surface on integrin α₅β₁–fibronectin interactions and the subsequent osteoblast behavior. Cation adsorption properties are closely associated with the extent of structural defects in materials. Here, the birnessite‐type MnO₂ coatings with different amounts of manganese vacancy (V_Mn) are prepared on Ti substrates via hydrothermal treatment (denoted as 1M100‐Ti, 5M100‐Ti, and 5M120‐Ti). The 5M120‐Ti surface with hierarchical flower‐like structure and the most stacked [MnO₆] layers possess the highest V_Mn content, which is determined by the ratios of Mn³⁺/Mn⁴⁺ and K/Mn. The increased V_Mn content enhances the Ca²⁺ and Mg²⁺ adsorption properties of the birnessite coatings. When immersed in Ca²⁺/Mg²⁺ aqueous solution, the birnessite coatings significantly promote fibronectin adsorption and the exposure of cell‐binding domains on adsorbed fibronectin with 5M120‐Ti showing the greatest enhancement. Accordingly, the 5M120‐Ti surface provides more α₅β₁‐integrin‐specific instructions to MC3T3‐E1 preosteoblasts capable of enhancing spreading and osteoblastic differentiation compared to two other samples. This study may provide a prospective approach of modulation of integrin–ligand interactions for controlling cell behavior by adjusting metal vacancies in metal oxides coatings.

中文翻译：

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通过富镁的水钠锰矿和纤连蛋白-整联蛋白相互作用的增强的二价阳离子调节来控制成骨细胞的行为。

成骨细胞功能相关联的整合素配体相互作用是已知的依赖于二价阳离子，但是存在关于Ca的效果稀缺信息²⁺和Mg ²⁺的整合素α植入物表面的吸附性质₅ β ₁ -fibronectin相互作用和随后的成骨细胞行为。阳离子的吸附性能与材料中结构缺陷的程度密切相关。在这里，锰矿量不同的水钠锰矿型MnO ₂涂层（V _Mn）是通过水热处理（分别表示为1M100-Ti，5M100-Ti和5M120-Ti）在Ti基片上制备的。的5M120-Ti系表面具有分级花状结构和最堆叠[MnO的₆ ]层拥有最高V_的Mn含量，这是由Mn的比率确定³⁺ / Mn为⁴⁺和K / Mn等。增加的V _Mn含量增强了水钠锰矿涂层的Ca ²⁺和Mg ²⁺吸附性能。浸入Ca ²⁺ / Mg ²⁺在水溶液中，水钠锰矿涂层显着促进了纤连蛋白的吸附，而5M120-Ti对吸附的纤连蛋白的细胞结合结构域的暴露显示出最大的增强作用。因此，5M120-Ti系表面提供了更多的α ₅ β ₁整合素特异性指令以能够增强相对于其他两个样品铺展和成骨细胞分化的MC3T3-E1前成骨细胞。这项研究可能通过调节金属氧化物涂层中的金属空位，提供了调节整联蛋白-配体相互作用以控制细胞行为的前瞻性方法。