Introduction: Respiratory diseases are leading causes of death in the world, still inhalation therapies are the largest fail in drug development. There is an evident need to develop new therapies. Biomolecules represent apotential therapeutic agent in this regard, however their translation to the clinic is hindered by the lack of tools to efficiently deliver molecules. Cell penetrating peptides (CPPs) have arisen as apotential strategy for intracellular delivery that could theoretically enable the translation of new therapies.Areas covered: In this review, the use of CPPs as astrategy to deliver different molecules (cargoes) to treat lung-relateddiseases will be the focus. Abrief description of these molecules and the innovative methods in designing new CPPs is presented. The delivery of different cargoes (proteins, peptides, poorly soluble drugs and nucleic acids) using CPPs is discussed, focusing on benefits to treat different respiratory diseases like inflammatory disorders, cystic fibrosis and lung cancer.Expert opinion: The advantages of using CPPs to deliver biomolecules and poorly soluble drugs to the lungs is evident. This field has advanced in the past few years toward targeted intracellular delivery, although further studies are needed to fully understand its potential and limitations in vitro and in vivo.

中文翻译：

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使用细胞穿透肽进行呼吸道药物输送方面的进展。

简介：呼吸系统疾病是世界上主要的死亡原因，吸入疗法仍然是药物开发的最大失败。显然需要开发新的疗法。在这方面，生物分子代表了潜在的治疗剂，但是由于缺乏有效递送分子的工具，生物分子在临床上的翻译受到了阻碍。细胞穿透肽（CPPs）作为细胞内递送的潜在策略已经出现，从理论上讲可以实现新疗法的翻译。研究领域：在本综述中，使用CPPs作为策略来递送不同的分子（货物）来治疗肺相关疾病成为焦点。简要介绍了这些分子以及设计新CPP的创新方法。运送不同的货物（蛋白质，多肽，讨论了使用CPPs的难溶性药物和核酸），重点讨论了治疗各种呼吸系统疾病（如炎症性疾病，囊性纤维化和肺癌）的益处专家意见：使用CPPs将生物分子和难溶性药物递送至肺部的优势显而易见。尽管需要进一步研究以充分了解其在体外和体内的潜力和局限性，但该领域在过去几年中已朝着靶向细胞内递送的方向发展。