In the present work, a novel platform has been designed to fabricate an electrochemical sensor composed of chitosan nanofibers modified with sodium dodecyl sulfate, a negatively charged surfactant, and hemoglobin protein for direct and sensitive determination of hydrogen peroxide. Chitosan nanofibers were synthesized through the electrospinning process and characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. The synthesized nanofibers provided a large surface for the adsorption of hemoglobin. Immobilization of hemoglobin on the fibers was investigated by cyclic voltammetry, and the modification steps were followed by electrochemical impedance spectroscopy. Under the optimal conditions, the fabricated biosensor was employed for electrocatalytic determination of hydrogen peroxide by differential pulse voltammetric and amperometric methods in the linear range 3.0–2940.0 µM (detection limit = 0.16 µM) and 1.0–201.0 µM (detection limit = 0.05 µM), respectively. The applicability of the constructed biosensor was also evaluated by hydrogen peroxide determination in real samples, and satisfactory recoveries were obtained which confirmed that the proposed method provides a simple and sensitive method for the hydrogen peroxide monitoring.

中文翻译：

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使用高性能平台进行生物电催化和直接测定H 2 O 2：经SDS和血红蛋白修饰的壳聚糖纳米纤维

在目前的工作中，已设计出一种新型平台来制造电化学传感器，该传感器由用十二烷基硫酸钠修饰的壳聚糖纳米纤维，带负电荷的表面活性剂和血红蛋白组成，用于直接和灵敏地测定过氧化氢。壳聚糖纳米纤维是通过静电纺丝工艺合成的，并通过傅里叶变换红外光谱和扫描电镜进行表征。合成的纳米纤维为吸附血红蛋白提供了较大的表面。通过循环伏安法研究了血红蛋白在纤维上的固定，并通过电化学阻抗谱进行了修饰步骤。在最佳条件下 所制造的生物传感器通过差分脉冲伏安法和安培法分别用于线性范围为3.0–2940.0 µM（检测极限= 0.16 µM）和1.0–201.0 µM（检测极限= 0.05 µM）的电催化测定过氧化氢。还通过测定实际样品中的过氧化氢来评估所构建生物传感器的适用性，并获得令人满意的回收率，这证实了所提出的方法为过氧化氢监测提供了一种简单而灵敏的方法。