Biomaterials may be useful in filling lost bone portions in order to restore balance and improve bone regeneration. The objective of this study was to produce polycaprolactone (PCL) membranes combined with two types of bioglass (Sol-Gel and melt-quenched) and determine their physical and biological properties. Membranes were produced through electrospinning. This study presented three experimental groups: pure PCL membranes, PCL-Melt-Bioglass and PCL-Sol-gel-Bioglass. Membranes were characterized using Scanning Electron Microscopy, Fourier Transform Infrared Spectrophotometry (FTIR), Energy-Dispersive Spectroscopy and Zeta Potential. The following in vitro tests were performed: MTT assay, alkaline phosphatase activity, total protein content and mineralization nodules. Twenty-four male rats were used to observe biological performance through radiographic, fracture energy, histological and histomorphometric analyses. The physical and chemical analysis results showed success in manufacturing bioactive membranes which significantly enhanced cell viability and osteoblast differentiation. The new formed bone from the in vivo experiment was similar to that observed in the control group. In conclusion, the electrospinning enabled preparing PCL membranes with bioglass incorporated into the structure and onto the surface of PCL fibers. The microstructure of the PCL membranes was influenced by the bioglass production method. Both bioglasses seem to be promising biomaterials to improve bone tissue regeneration when incorporated into PCL.

中文翻译：

---

PCL支架与生物玻璃的结合：合成，表征和生物学性能。

生物材料可用于填充失去的骨骼部分，以恢复平衡并改善骨骼再生。这项研究的目的是生产结合两种类型的生物玻璃（Sol-Gel和熔融淬火）的聚己内酯（PCL）膜，并确定其物理和生物学特性。膜是通过静电纺丝生产的。这项研究提出了三个实验组：纯PCL膜，PCL-熔融-生物玻璃和PCL-溶胶-凝胶-生物玻璃。使用扫描电子显微镜，傅立叶变换红外分光光度法（FTIR），能量色散光谱法和Zeta电位对膜进行表征。进行了以下体外测试：MTT分析，碱性磷酸酶活性，总蛋白含量和矿化结节。用二十四只雄性大鼠通过射线照相，骨折能量，组织学和组织形态分析来观察其生物学性能。物理和化学分析结果显示成功制造了生物活性膜，该膜显着增强了细胞活力和成骨细胞分化。来自体内实验的新形成的骨骼与对照组中观察到的相似。总之，静电纺丝能够制备将生物玻璃掺入结构和PCL纤维表面的PCL膜。PCL膜的微观结构受到生物玻璃生产方法的影响。当掺入PCL时，两种生物玻璃似乎都是有望改善骨组织再生的生物材料。物理和化学分析结果显示成功制造了生物活性膜，该膜显着增强了细胞活力和成骨细胞分化。来自体内实验的新形成的骨骼与对照组中观察到的相似。总之，静电纺丝能够制备将生物玻璃掺入结构和PCL纤维表面的PCL膜。PCL膜的微观结构受到生物玻璃生产方法的影响。当掺入PCL时，两种生物玻璃似乎都是有望改善骨组织再生的生物材料。物理和化学分析结果显示成功制造了生物活性膜，该膜显着增强了细胞活力和成骨细胞分化。来自体内实验的新形成的骨骼与对照组中观察到的相似。总之，静电纺丝能够制备将生物玻璃掺入结构和PCL纤维表面的PCL膜。PCL膜的微观结构受到生物玻璃生产方法的影响。当掺入PCL时，两种生物玻璃似乎都是有望改善骨组织再生的生物材料。来自体内实验的新形成的骨骼与对照组中观察到的相似。总之，静电纺丝能够制备将生物玻璃掺入结构和PCL纤维表面的PCL膜。PCL膜的微观结构受到生物玻璃生产方法的影响。当掺入PCL时，两种生物玻璃似乎都是有望改善骨组织再生的生物材料。来自体内实验的新形成的骨骼与对照组中观察到的相似。总之，静电纺丝能够制备将生物玻璃掺入结构和PCL纤维表面的PCL膜。PCL膜的微观结构受到生物玻璃生产方法的影响。当掺入PCL时，两种生物玻璃似乎都是有望改善骨组织再生的生物材料。