Pseudomonas putida and closely-related species such as Pseudomonas fluorescens and Pseudomonas brassicacearum have been reported as potential biocontrol agents and plant growth-promoters. Recently, we have described the biocontrol activity of P. putida B2017 against several phytopathogens of agricultural relevance. In this study, its ability to produce potential antibiotic / toxic metabolites was assessed by functional, chromatography-mass spectrometry and genomic analysis. Our results show that B2017 is not able to synthesize surfactants and common antibiotics produced by Pseudomonas spp., i.e. pyrrolnitrin, 2,4-diacetylphloroglucinol, pyoluteorin and pyocyanin, but it produces pyoverdine, a siderophore which is involved in its biocontrol activity. The non-production of other metabolites, such as cyanide, safracin, promysalin and lipopeptides between others, is also discussed. Our data suggest that the mode of action of B2017 is not mainly due to the production of antimicrobial / toxic metabolites. Moreover, these features make P. putida B2017 a promising biocontrol microorganism for plant protection without side effects on environment, non-target organisms and human health.

中文翻译：

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生物防治剂恶臭假单胞菌B2017没有产生抗生素和有毒代谢产物。

恶臭假单胞菌（Pseudomonas putida）和密切相关的物种如荧光假单胞菌（Pseudomonas fluorescens）和黄铜假单胞菌（Pseudomonas brasicacearum）已被报道为潜在的生物防治剂和植物生长促进剂。最近，我们描述了恶臭假单胞菌B2017对几种与农业相关的植物病原体的生物防治活性。在这项研究中，通过功能，色谱-质谱和基因组分析评估了其产生潜在抗生素/有毒代谢产物的能力。我们的结果表明，B2017不能合成假单胞菌属物种产生的表面活性剂和常见抗生素，即吡咯硝菌素，2,4-二乙酰基间苯三酚，pyoluteorin和pyyocyanin，但会生成吡over乙啶，这是一种参与其生物防治活性的铁载体。不产生其他代谢物，例如氰化物，沙弗拉明，还讨论了早孕素和脂肽之间的关系。我们的数据表明，B2017的作用方式主要不是由于产生了抗微生物/有毒代谢产物。此外，这些特性使恶臭假单胞菌B2017成为一种有前途的生物防治微生物，可用于植物保护，而不会对环境，非目标生物和人类健康产生副作用。