Trager and Jensen established a method for culturing Plasmodium falciparum, a breakthrough for malaria research worldwide. Since then, multiple attempts to establish Plasmodium vivax in continuous culture have failed. Unlike P. falciparum, which can invade all aged erythrocytes, P. vivax is restricted to reticulocytes. Thus, a constant supply of reticulocytes is considered critical for continuous P. vivax growth in vitro. A critical question remains why P. vivax selectively invades reticulocytes? What do reticulocytes offer to P. vivax that is not present in mature erythrocytes? One possibility is protection from oxidative stress by glucose-6-phosphate dehydrogenase (G6PD). Here, we also suggest supplements to the media and procedures that may reduce oxidative stress and, as a result, establish a system for the continuous culture of P. vivax.

中文翻译：

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培养间日疟原虫的一种方法。

Trager和Jensen建立了一种培养恶性疟原虫的方法，这是全球疟疾研究的一项突破。从那时起，在连续培养中建立间日疟原虫的多次尝试均告失败。与恶性疟原虫可以侵入所有老化的红细胞不同，间日疟原虫局限于网织红细胞。因此，网状细胞的恒定供应被认为对于体外间日疟原虫的连续生长至关重要。一个关键的问题仍然是，间日疟原虫为什么选择性地侵入网织红细胞？网状细胞为间日疟原虫提供了什么，而成熟红细胞中不存在？一种可能性是通过6-磷酸葡萄糖脱氢酶（G6PD）保护免受氧化应激。在这里，我们还建议补充培养基和程序，以减少氧化应激，从而建立间日疟原虫连续培养的系统。