Anoxic biotrickling filters (BTFs) represent a technology with high H2S elimination capacity and removal efficiencies widely studied for biogas desulfurization. Three changes in the final electron acceptors were made using nitrate and nitrite during an operating period of 520 days. The stability and performance of the anoxic BTF were maintained when a significant perturbation was applied to the system that involved the progressive change of nitrate to nitrite and vice versa. Here the impact of electron acceptor changes on the microbial community was characterized by denaturing gel gradient electrophoresis (DGGE) and next generation sequencing (NGS). Both platforms revealed that the community underwent changes during the perturbations but was resilient because the removal capacity did not significantly change. Proteobacteria and Bacteroidetes were the main Phyla and Sulfurimonas and Thiobacillus the main nitrate-reducing sulfide-oxidizing bacteria (NR-SOB) genera involved in the biodesulfurization process.

中文翻译：

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通过变性梯度凝胶电泳 (DGGE) 和下一代测序 (NGS) 在脱硫生物滴滤器中表征真细菌群落，使用硝酸盐和亚硝酸盐的渐进变化作为最终电子受体

缺氧生物滴滤器 (BTF) 是一种具有高 H2S 消除能力和去除效率的技术，被广泛研究用于沼气脱硫。在 520 天的运行期间，使用硝酸盐和亚硝酸盐对最终电子受体进行了三个变化。当对涉及硝酸盐和亚硝酸盐逐渐变化的系统施加显着扰动时，可以保持缺氧 BTF 的稳定性和性能。在这里，电子受体变化对微生物群落的影响以变性凝胶梯度电泳 (DGGE) 和下一代测序 (NGS) 为特征。两个平台都显示社区在扰动期间经历了变化，但由于清除能力没有显着变化而具有弹性。