Rational design of mimetic peptides based on the interaction between Inga laurina inhibitor and trypsins for Spodoptera cosmioides pest control.,Insect Biochemistry and Molecular Biology

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Rational design of mimetic peptides based on the interaction between Inga laurina inhibitor and trypsins for Spodoptera cosmioides pest control.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-04-29 , DOI: 10.1016/j.ibmb.2020.103390
Yaremis Meriño-Cabrera ₁ , José Gregório Severiche Castro ₂ , Juan Diego Rios Diez ₃ , Maria Ligia Rodrigues Macedo ₄ , Tiago Antônio de Oliveira Mendes ₁ , Maria Goreti de Almeida Oliveira ₁

Affiliation

The interaction of Inga laurina Kunitz inhibitor with insect trypsins is an example of protein-protein interaction with potential application for the pest control. However, the crop field application of proteins as inhibitors is limited due to high production cost, the large molecular size and low environmental stability. The use of mimetic peptides that have molecular features associated with the protein inhibitor can result in a product with lower cost and higher efficiency for the agricultural application. Here, we designed mimetic peptides deriving from globular domains of ILTI that are predicted to interact with trypsin enzymes of Lepidoptera pest. Two linear peptides were identified and synthetized from the interface of interaction between trypsin-ILTI complexes. These peptides were derived due to its high-energy contribution for the biding affinity between the enzyme-protein inhibitor. The peptides showed structural stability, propensity to adopt the bound conformation also without the context of the protein, inhibitory activity of digestive trypsins and toxic effects on the S. cosmioides, indicating that they can be used as potential inhibitor for pest control.

中文翻译：

基于Inga laurina抑制剂与胰蛋白酶之间相互作用的模拟肽的合理设计，用于防治夜蛾（Spodoptera cosmioides）害虫。

Inga laurina Kunitz抑制剂与昆虫胰蛋白酶的相互作用是蛋白质与蛋白质相互作用的一个例子，具有潜在的防治害虫的作用。然而，由于高生产成本，大分子尺寸和低环境稳定性，限制了蛋白质作为抑制剂在田间的田间应用。具有与蛋白质抑制剂相关的分子特征的模拟肽的使用可以产生具有较低成本和较高农业应用效率的产品。在这里，我们设计了源自ILTI球状结构域的模拟肽，该肽可与鳞翅目害虫的胰蛋白酶相互作用。从胰蛋白酶-ILTI复合物之间相互作用的界面中鉴定并合成了两个线性肽。这些肽是由于其对酶-蛋白质抑制剂之间的亲和力的高能贡献而衍生的。这些肽显示出结构稳定性，在没有蛋白质的情况下也倾向于采用结合构象的结构，消化胰蛋白酶的抑制活性以及对棉铃虫的毒性作用，这表明它们可用作潜在的害虫控制抑制剂。

更新日期：2020-04-29

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