Research question

Can organoids be established from endometrial tissue of infertile women and does tissue cryopreservation allow for establishment of organoids comparable to organoids derived from freshly biopsied endometrial tissue?

Design

Endometrial tissue was obtained from six infertile women through minimally invasive biopsy using a Pipelle catheter and subjected to organoid development, immediately after biopsy as well as after tissue cryopreservation. Organoid formation efficiency, morphology, expandability potential, endometrial marker expression (immunostaining and reverse transcription quantitative real-time polymerase chain reaction) and hormonal responsiveness (after oestradiol and progesterone treatment) were assessed.

Results

Organoids established from both fresh and frozen tissue at comparable efficiency could be passaged long-term and showed similar morphology, i.e. cystic with a central lumen lined by a single epithelial cell layer. They also exhibited comparable expression of endometrial markers and proliferative activity (Ki67 expression). Finally, organoids from freshly biopsied and cryopreserved endometrial tissue showed similar responses to oestradiol and progesterone treatment.

Conclusions

Organoids can be established from cryopreserved endometrial tissue of infertile women and cryopreservation of the biopsy does not affect organoid formation and overall organoid characteristics. Cryopreservation of biopsies for later organoid development facilitates sample collection from any fertility clinic, not just the ones near an organoid laboratory.

中文翻译：

---

可以从不育妇女的冷冻活检导管衍生的子宫内膜组织中可靠地建立类器官。

研究问题

能否从不育妇女的子宫内膜组织中建立类器官，组织冷冻保存是否允许建立与来自新鲜活检子宫内膜组织的类器官相当的类器官？

设计

使用 Pipelle 导管通过微创活检从 6 名不孕妇女身上获得子宫内膜组织，并在活检后和组织冷冻保存后立即进行类器官发育。评估了类器官形成效率、形态、扩展潜力、子宫内膜标志物表达（免疫染色和逆转录定量实时聚合酶链反应）和激素反应性（雌二醇和黄体酮治疗后）。

结果

从新鲜和冷冻组织中建立的类器官可以以相当的效率长期传代并表现出相似的形态，即具有中心腔内衬单个上皮细胞层的囊性。它们还表现出类似的子宫内膜标志物表达和增殖活性（Ki67 表达）。最后，来自新鲜活检和冷冻保存的子宫内膜组织的类器官对雌二醇和黄体酮治疗显示出相似的反应。

结论

类器官可以从不育女性的冷冻保存的子宫内膜组织中建立，活检的冷冻保存不会影响类器官的形成和整体类器官特征。为以后的类器官发育进行活检的冷冻保存有助于从任何生育诊所收集样本，而不仅仅是类器官实验室附近的诊所。