Decades of research have underscored the importance of cyclic nucleotide signaling in memory formation and synaptic plasticity. In recent years, several new genetic techniques have expanded the neuroscience toolbox, allowing researchers to measure and modulate cyclic nucleotide gradients with high spatiotemporal resolution. Here, we will provide an overview of studies using genetic approaches to interrogate the role cyclic nucleotide signaling plays in hippocampus-dependent memory processes and synaptic plasticity. Particular attention is given to genetic techniques that measure real-time changes in cyclic nucleotide levels as well as newly-developed genetic strategies to transiently manipulate cyclic nucleotide signaling in a subcellular compartment-specific manner with high temporal resolution.

数十年的研究强调了环状核苷酸信号在记忆形成和突触可塑性中的重要性。近年来，几种新的遗传技术扩展了神经科学工具箱，使研究人员能够以高时空分辨率测量和调节环状核苷酸梯度。在这里，我们将提供有关使用遗传方法询问循环核苷酸信号在海马依赖性记忆过程和突触可塑性中发挥作用的研究的概述。特别关注遗传技术，该技术可测量环状核苷酸水平的实时变化，以及新开发的遗传策略，可以以高时间分辨率以亚细胞区室特异性方式瞬时操纵环状核苷酸信号。