In this work, the CH₂Cl₂ phase from the EtOH extract of Oxandra sessiliflora (Annonaceae) branches was subjected to chromatographic fractionation to afford six sesquiterpenoids: 4α,10β-aromadendranediol (1), 4β,10α-aromadendranediol (2), 4α,10α-aromadendranediol (3), 1β,6α-dihydroxy-4(15)-eudesmene (4), 4β,10α-dihydroxy-guai-6-ene (5), and 4β,6β,7β,10α-tetrahydroxy-guaiane (6), the last one identified as a new natural product. The structures of isolated compounds were identified by analysis of NMR and HRESIMS spectral data. Compounds 1–6 exhibited activity against T. cruzi with EC₅₀ values ranging from 16.3–47.5 μM. Additionally, no cytotoxicity to mammalian cells (NCTC- L929 clone) was observed for tested compounds at the highest concentration (200 μM). Sesquiterpenes 5 and 6 exhibited higher selectivity index (SI) with values higher than 12.3 and 11.4, respectively. This data suggests that guaiane sesquiterpenes 5 and 6, isolated from O. sessiliflora, may contribute as scaffolds for the design of novel and selective drug candidates for treatment of neglected diseases, mainly for Chagas disease.

在这项工作中，对Ox桑（Oxandra sessiliflora（Annonaceae）分支的EtOH提取物的CH ₂ Cl ₂相进行色谱分离，从而得到六个倍半萜类化合物：4α，10β-aromadendranediol（1），4β，10α-aromadendranediol（2），4α ，10α-芳族马ran丹二醇（3），1β，6α-二羟基-4（15）-癸二烯（4），4β，10α-二羟基-gua -6-烯（5）和4β，6β，7β，10α-四羟基- guaiane（6），最后一个被确定为新的天然产物。通过分析NMR和HRESIMS光谱数据鉴定了分离的化合物的结构。化合物1–6对EC ₅₀值范围为16.3–47.5μM的T. cruzi。另外，对于最高浓度（200μM）的测试化合物，未观察到对哺乳动物细胞（NCTC-L929克隆）的细胞毒性。倍半萜5和6表现出更高的选择性指数（SI），其值分别高于12.3和11.4。该数据表明，从欧塞木（O. sessiliflora）中分离出的愈创树倍半萜5和6可能作为支架设计新的和选择性的候选药物，以治疗被忽视的疾病，主要是南美锥虫病。