Fermentation of carbohydrates present in lignocellulosic (LC) biomass is facilitated by lignin removal, which is usually achieved by adopting various pretreatment methods to provide the enzymes proper access to their respective substrates. Pretreatment using ionic liquid (IL) is relatively recent advancement and considered as mild and green process. ILs can dissolve extensive quantities of biomass and depolymerize the cellulose. In this context, an abundantly available LC biomass, sugarcane bagasse (SB), was pretreated using alkali or with an IL, methyltrioctylammonium chloride, and was used for cellulase production from thermophilic bacteria. In all, 26 indigenously isolated thermophilic bacterial strains were quantitatively screened for cellulase production. 16S rDNA sequences of the promising isolates UE10 and UE27 revealed relatedness with Brevibacillus borstelensis, while the strain UE1 belonged to Aneurinibacillus thermoaerophilus. Cellulase production was compared by utilizing alkali pretreated and IL pretreated SB and the later was found more appropriate. UE1, UE10 and UE27 yielded 22.2, 22.18 and 33.3 IU mL-1 of endoglucanase, respectively, by fermenting IL pretreated SB. The changes in SB structure after pretreatment were evaluated by scanning electron microscopy. This study demonstrated the potential of novel thermophilic bacterial strains to utilize IL pretreated SB for production of industrially important enzyme, cellulase.

中文翻译：

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甲基三辛基氯化铵作为新型离子液体在甘蔗渣预处理中用于新型嗜热细菌生产纤维素酶的研究。

木质素去除可促进木质纤维素（LC）生物质中存在的碳水化合物的发酵，这通常可通过采用各种预处理方法使酶正确进入其各自的底物来实现。使用离子液体（IL）进行预处理是相对较新的进展，被认为是温和而绿色的过程。IL可溶解大量生物质并使纤维素解聚。在这种情况下，使用碱或用IL甲基三辛基氯化铵对大量可用的LC生物质甘蔗渣（SB）进行了预处理，并用于由嗜热细菌生产纤维素酶。总共对26种本地分离的嗜热细菌菌株进行了纤维素酶生产的定量筛选。有希望的分离株UE10和UE27的16S rDNA序列显示出与博氏短杆菌（Brevibacillus borstelensis）的相关性，而菌株UE1属于嗜热嗜热芽孢杆菌。通过使用碱预处理的和IL预处理的SB比较纤维素酶的产量，发现后者更合适。UE1，UE10和UE27通过发酵IL预处理的SB分别产生22.2、22.18和33.3 IU mL-1的内切葡聚糖酶。通过扫描电子显微镜评价预处理后SB结构的变化。这项研究证明了新型嗜热细菌菌株利用IL预处理的SB生产工业上重要的酶纤维素酶的潜力。通过使用碱预处理的和IL预处理的SB来比较纤维素酶的产量，发现后者更合适。UE1，UE10和UE27通过发酵IL预处理的SB分别产生22.2、22.18和33.3 IU mL-1的内切葡聚糖酶。通过扫描电子显微镜评估预处理后SB结构的变化。这项研究证明了新型嗜热细菌菌株利用IL预处理的SB生产工业上重要的酶纤维素酶的潜力。通过使用碱预处理的和IL预处理的SB来比较纤维素酶的产量，发现后者更合适。UE1，UE10和UE27通过发酵IL预处理的SB分别产生22.2、22.18和33.3 IU mL-1的内切葡聚糖酶。通过扫描电子显微镜评价预处理后SB结构的变化。这项研究证明了新型嗜热细菌菌株利用IL预处理的SB生产工业上重要的酶纤维素酶的潜力。