Short-term allograft survival has improved among solid organ transplant (SOT) patients. An increasing number of SOT patients are prepared for re-transplantation because of chronic allograft failure. Lack of HLA typing or incomplete HLA typing of previous donors complicates pretransplant risk assessment, as repeated HLA mismatches may be missed. In addition, a complete HLA type of the donor is essential in the diagnosis of antibody-mediated rejection. We aimed to determine donor HLA types from allograft biopsies from kidney, heart and liver grafts.

Graft biopsies were obtained from 13 kidney, heart and liver transplanted patients. HLA typing was performed using q-PCR. Alleles of both donor and recipient origin were detected, and donor HLA type was concluded by deducting known HLA types of the recipient. For all 13 patients, we were able to determine mismatched donor HLA alleles from graft material. These results are promising, because they enable better individualized risk assessment.

实体器官移植（SOT）患者的短期同种异体移植存活率有所提高。由于慢性同种异体移植失败，越来越多的SOT患者准备进行再次移植。以前的供体缺乏HLA分型或HLA分型不完整会使移植前的风险评估变得复杂，因为可能会遗漏重复的HLA不匹配。另外，供体的完整HLA类型对于抗体介导的排斥反应的诊断至关重要。我们旨在确定来自肾脏，心脏和肝脏移植物的同种异体移植活检的供体HLA类型。

从13位肾脏，心脏和肝脏移植患者中获得了移植物活检。使用q-PCR进行HLA分型。检测到供体和受体来源的等位基因，并通过扣除受体的已知HLA类型得出供体HLA类型。对于全部13例患者，我们能够从移植物材料中确定不匹配的供体HLA等位基因。这些结果令人鼓舞，因为它们可以更好地进行个性化风险评估。