Heat stress (HS) seriously affects sow lactation performance and Long non-coding RNAs (lncRNAs) play vital roles in the regulation of transcription and post transcription. However, the mechanism of lncRNAs expression affecting lactation performance on the hypothalamus-pituitary-mammary axis of sows is still unclear. In this study, we performed RNA sequencing and bioinformatics analysis of the hypothalamus, pituitary, and mammary gland tissues of lactating sows under HS and thermal comfort. In total, the analysis identified 658, 6021, and 6745 differently expressed (DE) mRNAs, 26, 126, and 169 DE lncRNAs between comparison groups in the hypothalamus, pituitary, and mammary glands, respectively. The hormone genes and most DE mRNAs encoding heat shock protein were differently expressed in the HS group. In addition, 2, 60, and 86 pairs of DE lncRNAs and mRNAs correlation were observed in those tissues, respectively. Some lncRNAs may be involved in the regulation of lactation performance in the HS sows.

中文翻译：

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热应激下泌乳母猪下丘脑-垂体-乳腺轴的长链非编码RNA和mRNA分析。

热应激 (HS) 严重影响母猪泌乳性能，长链非编码 RNA (lncRNA) 在转录和转录后调控中起着至关重要的作用。然而，lncRNAs表达影响母猪下丘脑-垂体-乳腺轴泌乳性能的机制尚不清楚。在这项研究中，我们对 HS 和热舒适条件下泌乳母猪的下丘脑、垂体和乳腺组织进行了 RNA 测序和生物信息学分析。总体而言，该分析在下丘脑、垂体和乳腺的对照组之间分别确定了 658、6021 和 6745 个差异表达 (DE) mRNA，以及 26、126 和 169 个 DE lncRNA。HS组中激素基因和大多数编码热休克蛋白的DE mRNA表达不同。此外，2、60、在这些组织中分别观察到 86 对 DE lncRNA 和 mRNA 的相关性。一些 lncRNA 可能参与了 HS 母猪泌乳性能的调节。