Vaccine adjuvants induce host innate immune responses improving long-lasting adaptive immunity against vaccine antigens. In vitro models can be used to compare these responses between adjuvants and the infection targeted by the vaccine. We utilized transcriptomic profiling of an Atlantic salmon cell line to compare innate immune responses against ISAV and an experimental viral vaccine adjuvant: poly (I:C). Induction of interferon and interferon induced genes were observed after both treatments, but often with different amplitude and kinetics. Using KEGG ortholog database and available software from Bioconductor we could specify a complete bioinformatic pipeline for analysis of transcriptomic data from Atlantic salmon, a feature not previously available. We have identified important differences in the transcriptional profile of Atlantic salmon cells exposed to viral infection and a viral vaccine adjuvant candidate, poly (I:C). This report increases our knowledge of viral host-pathogen interaction in salmon and to which extent these can be mimicked by adjuvant compounds.

中文翻译：

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大西洋鲑鱼肾 (ASK) 细胞系中针对聚 (I:C) 和传染性鲑鱼贫血病毒 (ISAV) 的转录反应动力学。

疫苗佐剂诱导宿主先天免疫反应，改善针对疫苗抗原的持久适应性免疫。体外模型可用于比较佐剂和疫苗靶向感染之间的这些反应。我们利用大西洋鲑鱼细胞系的转录组学分析来比较针对 ISAV 和实验性病毒疫苗佐剂的先天免疫反应：poly (I:C)。两种处理后均观察到干扰素的诱导和干扰素诱导的基因，但通常具有不同的幅度和动力学。使用 KEGG ortholog 数据库和 Bioconductor 的可用软件，我们可以指定一个完整的生物信息学管道来分析大西洋鲑鱼的转录组数据，这是以前没有的功能。我们已经确定了暴露于病毒感染的大西洋鲑鱼细胞和候选病毒疫苗佐剂 poly (I:C) 的转录谱的重要差异。该报告增加了我们对鲑鱼中病毒宿主 - 病原体相互作用的了解，以及佐剂化合物在多大程度上可以模拟这些相互作用。