SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza.,Journal of Integrative Plant Biology

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SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-04-28 , DOI: 10.1111/jipb.12943
Changping Deng _{1,

2} , Yao Wang _{1,

3} , Fenfen Huang ₃ , Sunjie Lu ₁ , Limei Zhao ₁ , Xingyuan Ma ₂ , Guoyin Kai _{1,

3}

Affiliation

MYB transcription factors play vital roles in plant growth and metabolism. The phytohormone methyl jasmonate (MeJA) promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza, but the regulatory mechanism is poorly understood. Here, we identified the MeJA‐responsive R2R3‐MYB transcription factor gene SmMYB2 from a transcriptome library produced from MeJA‐treated S. miltiorrhiza hairy roots. SmMYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP98A14. SmMYB2 was highly expressed in the periderm of S. miltiorrhiza and SmMYB2 localized to the nucleus. Overexpressing SmMYB2 in S. miltiorrhiza hairy roots significantly increased the levels of salvianolic acids (including rosmarinic acid and salvianolic acid B) by upregulating salvianolic acid biosynthetic genes such as CYP98A14. SmMYB2 binds to the MYB‐binding motifs in the promoter of CYP98A14, as confirmed by a dual‐luciferase assay and electrophoretic mobility shift assays. Anthocyanin contents were significantly higher in SmMYB2‐overexpressing hairy root lines than the control, primarily due to the increased expression of CHI, DFR, and ANS. These findings reveal the novel regulatory role of SmMYB2 in MeJA‐mediated phenolic acid biosynthesis, providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network.

中文翻译：

SmMYB2促进药用丹参中丹酚酸的生物合成。

MYB转录因子在植物生长和代谢中起着至关重要的作用。植物激素茉莉酮酸甲酯（MeJA）促进药草丹参中酚酸的积累，但其调控机制尚不清楚。在这里，我们从由MeJA处理过的S. miltiorrhiza毛状根产生的转录组文库中鉴定了MeJA响应性R2R3-MYB转录因子基因SmMYB2。SmMYB2表达紧密的键酚酸生物合成基因包括表达相关CYP98A14。SmMYB2中的周皮呈高表达丹参和SmMYB2定位于细胞核。过度表达SmMYB2在丹参毛状根通过上调酚酸生物合成基因如显著增加的丹酸（包括迷迭香酸和酚酸B）的水平CYP98A14。SmMYB2与CYP98A14启动子中的MYB结合基序结合，已通过双荧光素酶测定法和电泳迁移率测定法证实。在过表达SmMYB2的毛状根系中，花色苷含量显着高于对照，这主要是由于CHI，DFR和ANS的表达增加。这些发现揭示了SmMYB2在MeJA介导的酚酸生物合成中的新型调节作用，为代谢工程提供了有用的靶基因，并为丹酚酸调节网络提供了参考。

更新日期：2020-04-28

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