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Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-04-27 , DOI: 10.1002/mbo3.1051 Aiqun Yu 1 , Yu Zhao 1 , Jian Li 1 , Shenglong Li 1 , Yaru Pang 1 , Yakun Zhao 1 , Cuiying Zhang 1 , Dongguang Xiao 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-04-27 , DOI: 10.1002/mbo3.1051 Aiqun Yu 1 , Yu Zhao 1 , Jian Li 1 , Shenglong Li 1 , Yaru Pang 1 , Yakun Zhao 1 , Cuiying Zhang 1 , Dongguang Xiao 1
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Fatty acid ethyl esters (FAEEs) are fatty acid‐derived molecules and serve as an important form of biodiesel. The oleaginous yeast Yarrowia lipolytica is considered an ideal host platform for the production of fatty acid‐derived products due to its excellent lipid accumulation capacity. In this proof‐of‐principle study, several metabolic engineering strategies were applied for the overproduction of FAEE biodiesel in Y. lipolytica. Here, chromosome‐based co‐overexpression of two heterologous genes, namely, PDC1 (encoding pyruvate decarboxylase) and ADH1 (encoding alcohol dehydrogenase) from Saccharomyces cerevisiae, and the endogenous GAPDH (encoding glyceraldehyde‐3‐phosphate dehydrogenase) gene of Y. lipolytica resulted in successful biosynthesis of ethanol at 70.8 mg/L in Y. lipolytica. The engineered Y. lipolytica strain expressing the ethanol synthetic pathway together with a heterologous wax ester synthase (MhWS) exhibited the highest FAEE titer of 360.8 mg/L, which is 3.8‐fold higher than that of the control strain when 2% exogenous ethanol was added to the culture medium of Y. lipolytica. Furthermore, a synthetic microbial consortium comprising an engineered Y. lipolytica strain that heterologously expressed MhWS and a S. cerevisiae strain that could provide ethanol as a substrate for the production of the final product in the final engineered Y. lipolytica strain was created in this study. Finally, this synthetic consortium produced FAEE biodiesel at a titer of 4.8 mg/L under the optimum coculture conditions.
中文翻译：
使用油性酵母解脂耶氏酵母可持续生产FAEE生物柴油。
脂肪酸乙酯（FAEE）是脂肪酸衍生的分子，是生物柴油的重要形式。油性酵母解脂耶氏酵母被认为是生产脂肪酸衍生产品的理想宿主平台，因为它具有出色的脂质蓄积能力。在这项原理验证研究中，将几种代谢工程策略用于解脂耶氏酵母中FAEE生物柴油的超量生产。在这里，来自酿酒酵母的两个异源基因，即PDC1（编码丙酮酸脱羧酶）和ADH1（编码醇脱氢酶）以及内源性GAPDH基于染色体的共表达的（编码甘油醛-3-磷酸脱氢酶）基因解脂亚罗威阿酵母导致的乙醇成功的生物合成在70.8 mg / L的在解脂亚罗威阿酵母。工程改造的解脂亚罗威阿酵母株与异源蜡酯合成酶（MhWS）一起表达乙醇合成途径表现出360.8毫克/升的最高滴度FAEE，其比对照菌株的3.8倍时2％的外源乙醇添加到解脂耶氏酵母的培养基中。此外，合成微生物财团包含异源表达MhWS和酿酒酵母的工程化解脂耶氏酵母菌株。在这项研究中，创造了可以提供乙醇作为最终工程解脂耶氏酵母菌株生产终产物底物的菌株。最后，在最佳共培养条件下，该合成财团生产的FAEE生物柴油的滴度为4.8 mg / L。
更新日期：2020-04-27
中文翻译：
使用油性酵母解脂耶氏酵母可持续生产FAEE生物柴油。
脂肪酸乙酯（FAEE）是脂肪酸衍生的分子，是生物柴油的重要形式。油性酵母解脂耶氏酵母被认为是生产脂肪酸衍生产品的理想宿主平台，因为它具有出色的脂质蓄积能力。在这项原理验证研究中，将几种代谢工程策略用于解脂耶氏酵母中FAEE生物柴油的超量生产。在这里，来自酿酒酵母的两个异源基因，即PDC1（编码丙酮酸脱羧酶）和ADH1（编码醇脱氢酶）以及内源性GAPDH基于染色体的共表达的（编码甘油醛-3-磷酸脱氢酶）基因解脂亚罗威阿酵母导致的乙醇成功的生物合成在70.8 mg / L的在解脂亚罗威阿酵母。工程改造的解脂亚罗威阿酵母株与异源蜡酯合成酶（MhWS）一起表达乙醇合成途径表现出360.8毫克/升的最高滴度FAEE，其比对照菌株的3.8倍时2％的外源乙醇添加到解脂耶氏酵母的培养基中。此外，合成微生物财团包含异源表达MhWS和酿酒酵母的工程化解脂耶氏酵母菌株。在这项研究中，创造了可以提供乙醇作为最终工程解脂耶氏酵母菌株生产终产物底物的菌株。最后，在最佳共培养条件下，该合成财团生产的FAEE生物柴油的滴度为4.8 mg / L。
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