Protective Antioxidant Effects of Amentoflavone and Total Flavonoids from Hedyotis Diffusa on H 2 O 2 ‐ induced HL‐O2 Cells through ASK1/p38 MAPK Pathway,Chemistry & Biodiversity

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Protective Antioxidant Effects of Amentoflavone and Total Flavonoids from Hedyotis Diffusa on H 2 O 2 ‐ induced HL‐O2 Cells through ASK1/p38 MAPK Pathway
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-06-17 , DOI: 10.1002/cbdv.202000251
Yun-Lan Li _{1,

2} , Xi Chen ₁ , Si-Qing Niu ₁ , Hong-Yan Zhou ₁ , Qing-Shan Li _{1,

2}

Affiliation

In this study, total flavonoids and total triterpenoid acid were extracted with ethyl acetate from Hedyotis diffusa Willd, and hepatoprotective activities of them and five compounds from total flavonoids against H2O2 induced hepatocyte damage on HL‐02 cells were determined. In particular, amentoflavone and total flavonoids had influence on the leakage of ALT, AST, LDH, the activities of SOD and the content of MDA. They effectively reduced the loss of MMP, the release of Cyt C, and then inhibited activation of caspase‐3/caspase‐9 cascade in hepatotoxic cells. The contents of ROS were significantly reduced to inhibit p38 in amentoflavone and flavonoids groups which decreased ASK1 and p‐p38 levels through increasing thioredoxin Trx1 and reductase TrxR1. These results suggesting that the antioxidant protection of amentoflavone and flavonoids might be reducing ROS to inhibit the H2O2‐induced upstream of pathway via increasing levels of Trx1 and TrxR1, which were pivotal in blocking the down streaming effectors of ASK1/p38 MAPK pathway and alleviating hepatotoxicity.

中文翻译：

白花蛇舌草中的阿曼黄酮和总黄酮通过 ASK1/p38 MAPK 通路对 H 2 O 2 诱导的 HL-O2 细胞的保护性抗氧化作用

本研究从白花蛇舌草中用乙酸乙酯提取总黄酮和总三萜酸，测定了它们和总黄酮中的5种化合物对H2O2诱导的HL-02细胞肝细胞损伤的保肝活性。尤其是杏仁黄酮和总黄酮对ALT、AST、LDH的渗漏、SOD的活性和MDA的含量有影响。它们有效地减少了 MMP 的损失、Cyt C 的释放，然后抑制了肝毒性细胞中 caspase-3/caspase-9 级联的激活。活性氧的含量显着降低，以抑制 amentoflavone 和黄酮类化合物组中的 p38，通过增加硫氧还蛋白 Trx1 和还原酶 TrxR1 来降低 ASK1 和 p-p38 的水平。

更新日期：2020-06-17

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