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Retrospective study of porcine circovirus 3 (PCV3) in swine tissue from Brazil (1967–2018)
Brazilian Journal of Microbiology ( IF 2.1 ) Pub Date : 2020-04-28 , DOI: 10.1007/s42770-020-00281-6
I L F Rodrigues 1 , A C M Cruz 1 , A E Souza 1 , F B Knackfuss 2 , C H C Costa 3 , R L Silveira 1, 4 , T X Castro 1
Affiliation  

In this study, we report the molecular diagnosis and retrospective study of porcine circovirus 3 (PCV3) in frozen and formalin-fixed paraffin-embedded swine tissues (FFPE) collected from 1967 to 2018 in southeastern Brazil (Espírito Santo and Rio de Janeiro states). Frozen tissues from 35 pigs and FFPE tissues from 143 pigs were tested by nested PCR, targeting the PCV3 partial capsid gene. Bidirectional sequencing of 16 positive samples was performed, followed by sequence analysis and haplotype networks. A total of 26/178 samples (14.6%) tested positive for PCV3: 14/35 (40%) frozen tissue and 12/143 (8.4%) FFPE tissue. PCV3 was detected in the 1960s, 1970s, 2000s, and 2010s with the characterization of types PCV3a and PCV3b. A star-like distribution was observed in the grid of haplotypes, with a low haplotype diversity and more recent dispersal of the virus. A total of 40% of asymptomatic animals considered fit for slaughter tested positive for PCV3. In conclusion, PCV3 DNA was detected over 51 years of study, prior to initial reports and, so far, the sample detected in 1967 is the oldest partial capsid sequence described. The circulation of two different genotypes was reported, suggesting more than one introduction event of this virus into Brazil. Moreover, taken together, our studies indicated an ancient origin of PCV3 and its circulation in asymptomatic animals in Brazilian herds.

中文翻译:

巴西猪组织中猪圆环病毒 3 (PCV3) 的回顾性研究 (1967–2018)

在这项研究中,我们报告了 1967 年至 2018 年在巴西东南部(圣埃斯皮里图和里约热内卢州)收集的冷冻和福尔马林固定石蜡包埋猪组织 (FFPE) 中猪圆环病毒 3 (PCV3) 的分子诊断和回顾性研究. 35 头猪的冷冻组织和 143 头猪的 FFPE 组织通过巢式 PCR 检测,靶向 PCV3 部分衣壳基因。对 16 个阳性样本进行双向测序,然后进行序列分析和单倍型网络。总共 26/178 个样本 (14.6%) 检测出 PCV3 呈阳性:14/35 (40%) 冷冻组织和 12/143 (8.4%) FFPE 组织。在 1960 年代、1970 年代、2000 年代和 2010 年代检测到 PCV3,具有 PCV3a 和 PCV3b 型特征。在单倍型网格中观察到星状分布,具有低单倍型多样性和最近的病毒传播。共有 40% 的无症状动物被认为适合屠宰,PCV3 检测呈阳性。总之,PCV3 DNA 在最初报告之前的 51 年研究中被检测到,到目前为止,1967 年检测到的样本是所描述的最古老的部分衣壳序列。报告了两种不同基因型的传播,表明该病毒传入巴西的事件不止一次。此外,总的来说,我们的研究表明 PCV3 的古老起源及其在巴西畜群中无症状动物中的传播。1967 年检测到的样本是所描述的最古老的部分衣壳序列。报告了两种不同基因型的传播,表明该病毒传入巴西的事件不止一次。此外,总的来说,我们的研究表明 PCV3 的古老起源及其在巴西畜群中无症状动物中的传播。1967 年检测到的样本是所描述的最古老的部分衣壳序列。报告了两种不同基因型的传播,表明该病毒传入巴西的事件不止一次。此外,总的来说,我们的研究表明 PCV3 的古老起源及其在巴西畜群中无症状动物中的传播。
更新日期:2020-04-28
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