当前位置: X-MOL 学术Braz. J. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A sensitive and rapid bacterial antibiotic susceptibility test method by surface enhanced Raman spectroscopy
Brazilian Journal of Microbiology ( IF 2.1 ) Pub Date : 2020-04-28 , DOI: 10.1007/s42770-020-00282-5
Shijie Fu 1 , Xiwen Wang 2, 3 , Ting Wang 4 , Zhiping Li 5 , Deming Han 1 , Chunsheng Yu 6 , Cui Yang 6 , Han Qu 5 , Hang Chi 5 , Yutian Wang 5 , Song Li 1 , Baihui Tian 1 , Wenliang Li 7 , Zhiping Xia 5
Affiliation  

This study aims to develop a rapid bacterial antibiotic susceptibility test (AST) method by Bacteria-aptamer@AgNPs–surface enhanced Raman spectroscopy (SERS) and further evaluate the influence of different antibiotics on the Raman intensity of bacteria. The Raman intensity of Escherichia coli O157:H7 ( E. coli O157:H7) and Staphylococcus aureus ( S. aureus ) in the presence of different concentrations of antibiotics in 2 h was detected by Bacteria-aptamer@AgNPs-SERS in this study. Our results found that the bacteria Raman signal peak at 735 cm −1 and the minimum inhibitory concentration (MIC) value was determined in 1 h according to Raman signals. In 2 h, the bacteria Raman signal growth at sub-MIC concentrations of four different kinds of antibiotics and the bacteria colony-forming unit (CFU) have similar enhancements. SERS utilizes special functions of rough metal surfaces and offers a huge enhancement of Raman intensities with reduced fluorescence backgrounds, which makes it an ultrasensitive tool of detection. This rapid AST method and the enhancement effect should be of value in search of new antibiotic drugs.

中文翻译:

基于表面增强拉曼光谱的灵敏快速细菌抗生素药敏试验方法

本研究旨在通过细菌-适体@AgNPs-表面增强拉曼光谱(SERS)开发一种快速细菌抗生素药敏试验(AST)方法,并进一步评估不同抗生素对细菌拉曼强度的影响。本研究通过Bacteria-aptamer@AgNPs-SERS检测大肠杆菌O157:H7(E.coliO157:H7)和金黄色葡萄球菌(S.aureus)在不同浓度抗生素存在下2h的拉曼强度。我们的结果发现,细菌拉曼信号在 735 cm -1 处达到峰值,并且根据拉曼信号在 1 小时内确定了最小抑菌浓度 (MIC) 值。在 2 小时内,四种不同抗生素在亚 MIC 浓度下的细菌拉曼信号增长和细菌菌落形成单位 (CFU) 也有类似的增强。SERS 利用粗糙金属表面的特殊功能,在降低荧光背景的情况下极大地增强拉曼强度,使其成为一种超灵敏的检测工具。这种快速的 AST 方法和增强效果应该对寻找新的抗生素药物有价值。
更新日期:2020-04-28
down
wechat
bug