Due to increasing oil prices and climate change concerns, biofuels have become increasingly important as potential alternative energy sources. However, the use of arable lands and valuable resources for the production of biofuel feedstock compromises food security and negatively affect the environment. Single cell oils (SCOs), accumulated by oleaginous yeasts, show great promise for efficient production of biofuels. However, the high production costs attributed to feedstocks or raw materials present a major limiting factor. The fermentative conversion of abundant, low-value biomass into microbial oil would alleviate this limitation. Here, we explore the feasibility of utilizing microalgae-based cell residues as feedstock for yeast oil production. We developed an efficient, single-step enzymatic hydrolysis to generate Scenedesmus obtusiusculus hydrolysate (SH) without thermo-chemical pretreatment. With this eco-friendly process, glucose conversion efficiencies reached 90-100%. Cutaneotrichosporon oleaginosus, Cryptococcus curvatus and Rhodosporidium toruloides were cultivated on SH as sole nutrients source. Only C. oleaginosus was able to accumulate intracellular lipids, with a 35% (g lipid/g DCW) content and a yield of 3.6 g/L. Our results demonstrate the potential valorization of algal biomass into desired end-products such as biofuels.

中文翻译：

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钝角藻微藻生物质水解产物上生长的含油酵母产生的微生物脂质。

由于石油价格上涨和对气候变化的担忧，生物燃料作为潜在的替代能源变得越来越重要。但是，将耕地和宝贵资源用于生产生物燃料原料会损害粮食安全并给环境带来负面影响。含油酵母积累的单细胞油（SCO）显示出有效生产生物燃料的巨大希望。然而，归因于原料或原料的高生产成本是主要的限制因素。将丰富的低价值生物质发酵转化为微生物油将缓解这一限制。在这里，我们探讨了利用微藻类细胞残渣作为酵母油生产原料的可行性。我们开发了一种高效，单步酶解无需热化学预处理即可生成钝角腐殖质水解物（SH）。通过这种环保工艺，葡萄糖转化效率达到了90-100％。在SH上培养了角皮猪油菌，弯形隐球菌和环孢假单胞菌作为唯一的营养源。只有油酸梭菌能够积累细胞内脂质，含量为35％（克脂质/克DCW），产量为3.6克/升。我们的结果证明了将藻类生物质转化为所需最终产品（如生物燃料）的潜在价值。油橄榄能够积累细胞内脂质，含量为35％（克脂质/克DCW），产量为3.6克/升。我们的结果证明了将藻类生物质转化为所需最终产品（如生物燃料）的潜在价值。油橄榄能够积累细胞内脂质，含量为35％（克脂质/克DCW），产量为3.6克/升。我们的结果证明了藻类生物质转化为所需最终产品（如生物燃料）的潜在价值。