Hydrolytic enzymes play a significant role in hydrolyzing lignocellulosic polysaccharides into fermentable sugars. Due to the complex structure of lignocellulosic materials, developing robust processes for high titer production is a major challenge. The present study was investigated on xylanase production by filamentous fungi Aspergillus fumigatus strain JCM 10253 using agricultural residue ragi husk as a substrate for microbial fermentation. Central composite design (CCD) was employed to optimize the independent process variables and their effect on xylanase activity. Experimental results with optimized process variables showed 156.7 IU/mL activity under 1.24% substrate concentration and pH 2.0 at 49.9 °C after 120 h of the incubation period. The morphological study of isolate JCM 10253 was observed by scanning electron microscopy analysis and Fourier transform infrared spectroscopy was used to characterize the crude enzyme for secondary protein helical structures. Thus, this study demonstrates that ragi husk could be a potential substrate for xylanase production and can be further used as value-added industrial products.

水解酶在将木质纤维素多糖水解为可发酵糖中起着重要作用。由于木质纤维素材料的复杂结构，开发高滴定度生产的可靠工艺是一项重大挑战。本研究对丝状真菌烟曲霉生产木聚糖酶进行了研究。菌株JCM 10253，使用农业残留物ragi壳作为微生物发酵的底物。中央复合设计（CCD）用于优化独立的过程变量及其对木聚糖酶活性的影响。经过优化的工艺变量的实验结果显示，孵育120小时后，在1.24％底物浓度和pH 2.0于49.9°C下，活性为156.7 IU / mL。通过扫描电子显微镜分析观察到分离物JCM 10253的形态学，并使用傅里叶变换红外光谱法表征二级蛋白质螺旋结构的粗酶。因此，这项研究表明，拉吉果皮可能是木聚糖酶生产的潜在底物，并可以进一步用作增值工业产品。