Staphylococcus aureus demonstrates considerable membrane lipid plasticity in response to different growth environments, which is of potential relevance to response and resistance to various antimicrobial agents. This information is not available for various species of coagulase-negative staphylococci, which are common skin inhabitants, can be significant human pathogens, and are resistant to multiple antibiotics. We determined the total fatty acid compositions of Staphylococcus auricularis, Staphylococcus capitis, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus saprophyticus, and Staphylococcus aureus for comparison purposes. Different proportions of branched-chain and straight-chain fatty acids were observed amongst the different species. However, growth in cation-supplemented Mueller-Hinton broth significantly increased the proportion of branched-chain fatty acids, and membrane fluidities as measured by fluorescence anisotropy. Cation-supplemented Mueller-Hinton broth is used for routine determination of antimicrobial susceptibilities. Growth in serum led to significant increases in straight-chain unsaturated fatty acids in the total fatty acid profiles, and decreases in branched-chain fatty acids. This indicates preformed fatty acids can replace biosynthesized fatty acids in the glycerolipids of coagulase-negative staphylococci, and indicates that bacterial fatty acid biosynthesis system II may not be a good target for antimicrobial agents in these organisms. Even though the different species are expected to be exposed to skin antimicrobial fatty acids, they were susceptible to the major skin antimicrobial fatty acid sapienic acid (C16:1Δ6). Certain species were not susceptible to linoleic acid (C18:2Δ9,12), but no obvious relationship to fatty acid composition could be discerned.

中文翻译：

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凝固酶阴性葡萄球菌膜脂肪酸组成的可塑性及其对抗菌药物反应的影响。


金黄色葡萄球菌在不同的生长环境下表现出相当大的膜脂可塑性，这与对各种抗菌药物的反应和耐药性具有潜在的相关性。该信息不适用于各种凝固酶阴性葡萄球菌，它们是常见的皮肤居民，可能是重要的人类病原体，并且对多种抗生素具有抗药性。我们测定了耳状葡萄球菌、头状葡萄球菌、表皮葡萄球菌、溶血葡萄球菌、人葡萄球菌、腐生葡萄球菌和金黄色葡萄球菌的总脂肪酸组成，以进行比较。在不同物种中观察到支链和直链脂肪酸的比例不同。然而，补充阳离子的 Mueller-Hinton 肉汤中的生长显着增加了支链脂肪酸的比例，以及通过荧光各向异性测量的膜流动性。添加阳离子的 Mueller-Hinton 肉汤用于抗菌药物敏感性的常规测定。血清中的生长导致总脂肪酸谱中直链不饱和脂肪酸显着增加，而支链脂肪酸减少。这表明预形成的脂肪酸可以替代凝固酶阴性葡萄球菌甘油脂中的生物合成脂肪酸，并表明细菌脂肪酸生物合成系统II可能不是这些生物体中抗菌剂的良好靶标。尽管预计不同物种会接触皮肤抗菌脂肪酸，但它们对主要皮肤抗菌脂肪酸 sapienic Acid (C16:1Δ6) 很敏感。 某些物种对亚油酸 (C18:2Δ9,12) 不敏感，但与脂肪酸组成没有明显的关系。