Beginning in the 1960's, sowthistle yellow vein virus (SYVV) was the subject of pioneering research that demonstrated propagation of a plant virus in its insect vector. Since the 1980's there has been a paucity of research on SYVV, with historic isolates no longer maintained and no genomic sequence available. Once commonly observed infecting sowthistle (Sonchus oleraceous L.) in California, SYVV incidence declined ca. 1990, likely due to displacement of the black currant aphid (Hyperomyzus lactucae L.) by an invasive non-vector aphid. In 2018, SYVV was fortuitously rediscovered infecting sowthistle in an organic citrus grove in Kern County, CA. The SYVV genome sequence (13,719 nts) obtained from the 2018 sample (designated HWY65) encoded all six expected genes: N, P, MP, M, G, and L. Nucleotide sequence (representing ∼86 % of the genome) of the SYVV Berkeley lab isolate, used by E. S. Sylvester and colleagues for the paradigm-shifting research mentioned above, was determined from an archived library of cDNA clones constructed in 1986. The two nucleotide sequences share 98.5 % identity, confirming both represent the same virus, thereby linking biology of the historic isolate with extant SYVV rediscovered in 2018. Phylogenetic analysis of the L protein indicated SYVV is positioned within a clade containing a subset of viruses currently assigned to the genus Nucleorhabdovirus. As Nucleorhabdovirus is paraphyletic, the International Committee on the Taxonomy of Viruses has proposed abolishment of the genus and establishment of three new genera. In this revised taxonomy, the clade containing SYVV constitutes a new genus designated Betanucleorhabdovirus.

中文翻译：

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失物招领：在 30 多年的中断后重新发现和基因组特征的 sowthistle 黄脉病毒。

从 1960 年代开始，sowthistle 黄脉病毒 (SYVV) 是开创性研究的主题，证明了植物病毒在其昆虫载体中的繁殖。自 1980 年代以来，对 SYVV 的研究很少，历史分离株不再保留，也没有可用的基因组序列。一旦在加利福尼亚州普遍观察到感染猪蓟（Sonchus olerously L.），SYVV 发病率下降了约 1990 年，可能是由于侵入性非媒介蚜虫取代了黑醋栗蚜虫 (Hyperomyzus lactucae L.)。2018 年，在加利福尼亚州克恩县的有机柑橘园中，偶然地重新发现了 SYVV。从 2018 年样本（指定为 HWY65）获得的 SYVV 基因组序列（13,719 nts）编码了所有六个预期基因：N、P、MP、M、G 和 L。ES Sylvester 及其同事用于上述范式转换研究的 SYVV Berkeley 实验室分离株的核苷酸序列（代表约 86% 的基因组）是从 1986 年构建的 cDNA 克隆存档文库中确定的。 两个核苷酸序列共享 98.5 % 的同一性，确认两者代表相同的病毒，从而将历史分离株的生物学与 2018 年重新发现的现存 SYVV 联系起来。 L 蛋白的系统发育分析表明 SYVV 位于一个进化枝中，该进化枝包含目前分配给核棒状病毒属的病毒子集. 由于核棒状病毒属并系群，国际病毒分类委员会提议废除该属并建立三个新属。在这个修订后的分类法中，