Objective

To compare effectiveness of ethylenediaminetetraacetic acid (EDTA) and citric acid in removing collagen fiber network covering dentinal tubules of human teeth.

Materials and Methods

Eighteen dentin discs were divided in three groups; Gp 1: discs received no treatment (control), Gp 2: discs etched with 17% EDTA (pH = 7.1), and Gp 3: discs etched with 6 wt% citric acid (pH = 4.0). Scanning electron microscopy (SEM) was performed to assess collagen fiber removal and X-ray diffraction (XRD) was implemented to analyse crystal peaks of discs.

Results

The SEM analysis demonstrated more collagen removal with EDTA treatment compared to citric acid treated specimens. Grade 6 (81% to 100% fiber removal) was mostly achieved for Gp 2 samples whereas grade 2 (1% to 20% fiber removal) was mostly achieved for Gp 3 samples and inter-group comparisons between these groups were statistically significant (p < 0.05). X-ray diffractogram of control and experimental samples demonstrated absence of calcite phase in experimental groups. The change in peak shapes and intensities were observed and citric acid treated samples revealed more intense peaks than EDTA group.

Conclusion

Our study found 17% EDTA to be more effective in removing collagen fibers when matched with 6% citric acid.

中文翻译：

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两种螯合剂在牙本质小管胶原纤维网络修饰中的比较评价：体外分析。

客观的

比较乙二胺四乙酸（EDTA）和柠檬酸去除覆盖人牙牙本质小管的胶原纤维网络的效果。

材料和方法

十八个牙本质盘被分成三组；Gp 1：圆盘未接受处理（对照），Gp 2：用 17% EDTA（pH = 7.1）蚀刻的圆盘，和 Gp 3：用 6 wt% 柠檬酸（pH = 4.0）蚀刻的圆盘。进行扫描电子显微镜 (SEM) 以评估胶原纤维的去除，并实施 X 射线衍射 (XRD) 以分析圆盘的晶体峰。

结果

SEM 分析表明，与柠檬酸处理的样品相比，EDTA 处理的胶原蛋白去除率更高。Gp 2 样品主要达到 6 级（81% 至 100% 纤维去除），而 Gp 3 样品主要达到 2 级（1% 至 20% 纤维去除），并且这些组之间的组间比较具有统计学意义（p < 0.05)。对照和实验样品的 X 射线衍射图表明实验组中不存在方解石相。观察到峰形和强度的变化，柠檬酸处理的样品显示出比 EDTA 组更强烈的峰。

结论

我们的研究发现，当与 6% 的柠檬酸搭配使用时，17% 的 EDTA 可以更有效地去除胶原纤维。