Coxsackie B viruses (CV-B) are usually transmitted via the fecal-oral route and the virus gains the central nervous system (CNS) via the bloodstream. Nevertheless, other routes of spread of the virus to the CNS cannot be excluded, including the neuronal route. Neuronal cells, as well as non-neuronal cells (fibroblasts), were isolated from mice and inoculated with CV-B4 in the absence and presence of neutralizing serum. In the absence of neutralizing serum, virus titers recorded in neuron cultures and rates of infected neurons were non-significantly different compared to those recorded in fibroblast cultures. Higher cell mortality was noted among neurons than fibroblasts. The addition of neutralizing serum to neurons did not reduce significantly virus titers or rates of infected cells and cell viability was not significantly augmented, while virus titers and rates of infected fibroblasts were significantly reduced and their viability was significantly enhanced as well. Our results demonstrate the ineffectiveness of neutralizing serum to prevent neurons infection with CV-B4 which suggests a trans-synaptic transmission of CV-B4 between neurons.

中文翻译：

---

柯萨奇病毒B4感染和神经元间传播在原代培养的神经元中。

柯萨奇B病毒（CV-B）通常是通过粪-口途径传播的，该病毒通过血液获得中枢神经系统（CNS）。但是，不能排除病毒传播到中枢神经系统的其他途径，包括神经元途径。从小鼠分离神经元细胞以及非神经元细胞（成纤维细胞），并在不存在和存在中和血清的情况下用CV-B4接种。在没有中和血清的情况下，与在成纤维细胞培养物中记录的病毒滴度和感染神经元的速率相比，神经元培养物中记录的病毒滴度没有显着差异。注意到神经元中的细胞死亡率高于成纤维细胞。向神经元中加入中和血清不会显着降低病毒滴度或感染细胞的速度，并且细胞活力不会显着提高，病毒滴度和成纤维细胞感染率显着降低，它们的生存能力也显着提高。我们的结果证明中和血清无法预防CV-B4感染神经元，这提示了CV-B4在神经元之间的跨突触传递。