Effect of amino acids on IGF1 gene expression in human myotubes and skeletal muscle.,Growth Hormone and IGF Research

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Effect of amino acids on IGF1 gene expression in human myotubes and skeletal muscle.
Growth Hormone and IGF Research ( IF 1.6 ) Pub Date : 2020-04-28 , DOI: 10.1016/j.ghir.2020.101323
Egor M Lednev ₁ , Irina V Kravchenko ₂ , Vladimir A Furalyov ₂ , Evgeny A Lysenko ₁ , Iulia S Lemesheva ₁ , Alexandr A Grushin ₃ , Vadim E Dubrov ₄ , Olga L Vinogradova ₁ , Daniil V Popov ₁

Affiliation

Objective

Insulin-like growth factor I (IGF1) is an important regulator of collagen and extracellular matrix protein expression. We aimed to evaluate the effect of amino acids (AAs) on expression of IGF1 and IGF1-dependent genes in human myotubes and skeletal muscle and supposed that AAs administration increases IGF1 levels in blood and expression of IGF1 and IGF1-dependent genes in trained skeletal muscle, thereby reducing training-induced muscle damage.

Design

Human myotubes were incubated with Arg and Leu for 24 h. Then, the effects of long-term branched chain AAs administration (10 weeks, 0.1 g/kg body mass/day) to volunteers (six subjects per AAs and placebo groups) performing large training volumes regularly (cross country skiers, training twice a day) were examined.

Results

Incubating the myotubes with AAs increases expression of IGF1 mRNA isoforms and IGF1 secretion by 2–3 times. In athletes, long-term AAs administration increased basal blood levels of IGF1 (~50%) and expression of IGF1Ea mRNA slightly in skeletal muscle. There is no marked increase in expression of COL1A1, COL3A1, COL5A1, and LOX genes in skeletal muscle after AAs administration. However, expression of these genes in the combined group (placebo + AAs; n = 12) significantly correlated with the expression of IGF1Ea mRNA in muscle and did not correlate with IGF1 levels in the blood.

Conclusions

AAs administration increases IGF1 expression in vitro and in vivo. To obtain more pronounced changes in expression of IGF1 and IGF1-dependent genes in skeletal muscle, it may be necessary to increase the dose and/or duration of AAs administration.

中文翻译：

氨基酸对人肌管和骨骼肌 IGF1 基因表达的影响。

客观的

胰岛素样生长因子 I (IGF1) 是胶原蛋白和细胞外基质蛋白表达的重要调节剂。我们旨在评估氨基酸 (AAs) 对人肌管和骨骼肌中IGF1和 IGF1 依赖性基因表达的影响，并假设 AA 给药会增加血液中 IGF1 的水平以及训练后骨骼肌中IGF1和 IGF1 依赖性基因的表达，从而减少训练引起的肌肉损伤。

设计

人肌管与精氨酸和亮氨酸孵育 24 小时。然后，长期支链 AA 给药（10 周，0.1 g/kg 体重/天）对定期进行大量训练的志愿者（每个 AA 和安慰剂组六名受试者）（越野滑雪者，每天训练两次）的影响) 进行了检查。

结果

用 AA 孵育肌管可使IGF1 mRNA 同种型的表达和 IGF1 分泌增加 2-3 倍。在运动员中，长期服用 AA 会增加骨骼肌中 IGF1 的基础血液水平（~50%）和IGF1Ea mRNA 的表达。AA 给药后，骨骼肌中COL1A1、COL3A1、COL5A1和LOX基因的表达没有显着增加。然而，这些基因在组合组（安慰剂 + AA；n = 12）中的表达与肌肉中IGF1Ea mRNA的表达显着相关，而与血液中的 IGF1 水平无关。