The bathypelagic zone is a large, dark, cold, high-pressure environment that undergoes extensive material and energy exchanges with the upper ocean and the deep biosphere. Viruses are important parts of the deep ocean, and transportation of surface viruses has been considered as an important source of deep-sea viruses. However, limited information is available about the activity and decay of surface viruses in the deep-sea ecosystem. Therefore, this study investigated the stability of representative viral isolates of three morphological types with different sizes infecting autotrophic and heterotrophic bacteria by incubation in the dark under high hydrostatic pressure (HHP) of 30 MPa (corresponding to the pressure at a depth of about 3000 m) and 4 °C for two years. Cyanophage S-CBP1 and two roseophages, RDCBΦ1 and vB_DshS-R4C, were able to infect their hosts after long-term incubation under HHP. However, cyanophages S-CBWM1 and S-SCSM1, as well as roseophages RDCBΦ3 and vB_DshP-R2C, decayed and/or inactivated completely. The sensitivity of viral isolates to HHP might be related to their morphological characteristics, especially their capsid sizes. In addition, HHP treatment of environmental viral populations showed 92.2% reduction of low-fluorescence viruses and total loss of high-fluorescence viruses. The first experimental evidence from this study showed a large variability in the pressure effects on the stability and infectivity of different viruses and suggests that viruses isolated from the ocean's surface may survive under deep-sea conditions.

蓝海带是一个大型，黑暗，寒冷，高压的环境，与上层海洋和深层生物圈进行广泛的物质和能量交换。病毒是深海的重要组成部分，表面病毒的运输被认为是深海病毒的重要来源。但是，关于深海生态系统中表面病毒的活动和衰减的信息有限。因此，本研究通过在30 MPa的高静水压（HHP）下在黑暗中孵育（对应于约3000 m深度的压力），研究了三种不同大小的形态学代表病毒分离株感染自养和异养细菌的稳定性。 ）和4°C的温度保持两年。噬菌体S-CBP1和两个噬​​菌体RDCBΦ1和vB_DshS-R4C 在HHP下长期孵育后能够感染宿主。但是，蓝藻S-CBWM1和S-SCSM1以及迷迭香RDCBΦ3和vB_DshP-R2C完全腐烂和/或失活。病毒分离株对HHP的敏感性可能与其形态特征，特别是衣壳大小有关。此外，HHP处理环境病毒种群显示低荧光病毒减少了92.2％，高荧光病毒完全消失。这项研究的第一个实验证据表明，压力对不同病毒的稳定性和传染性的影响存在很大差异，并表明从海洋表面分离出的病毒可以在深海条件下生存。以及迷迭香RDCBΦ3和vB_DshP-R2C完全衰减和/或失活。病毒分离株对HHP的敏感性可能与其形态特征，特别是衣壳大小有关。此外，HHP处理环境病毒种群显示低荧光病毒减少了92.2％，高荧光病毒完全消失。这项研究的第一个实验证据表明，压力对不同病毒的稳定性和传染性的影响存在很大差异，并表明从海洋表面分离出的病毒可以在深海条件下生存。以及迷迭香RDCBΦ3和vB_DshP-R2C完全衰减和/或失活。病毒分离株对HHP的敏感性可能与其形态特征，特别是衣壳大小有关。此外，HHP处理环境病毒种群显示低荧光病毒减少了92.2％，高荧光病毒完全消失。这项研究的第一个实验证据表明，压力对不同病毒的稳定性和传染性的影响存在很大差异，并表明从海洋表面分离出的病毒可以在深海条件下生存。HHP处理环境病毒种群显示低荧光病毒减少了92.2％，高荧光病毒完全消失。这项研究的第一个实验证据表明，压力对不同病毒的稳定性和传染性的影响存在很大差异，并表明从海洋表面分离出的病毒可以在深海条件下生存。HHP处理环境病毒种群显示低荧光病毒减少了92.2％，高荧光病毒完全消失。这项研究的第一个实验证据表明，压力对不同病毒的稳定性和传染性的影响存在很大差异，并表明从海洋表面分离出的病毒可以在深海条件下生存。