Gene expression profiling in mouse models of leishmaniasis has given useful information to understand the molecular pathways active in lesions and to discover new diagnostic/therapeutic targets. Although the host response plays a critical role in protection from leishmaniasis and promoting disease severity, there are still unexplained aspects in the mechanism of non-healing cutaneous lesions, which need biomarkers for both targeted- therapy and diagnosis. To address this, transcriptional profiling of the skin lesions obtained from BALB/c mice infected with Leishmania major and healthy skin from naïve mice were evaluated by bioinformatics analysis, and then the results were validated by Revers Transcriptase-PCR. Five genes among the up-regulated differentially expressed genes named FCGR4, CCL4, CXCL9, Arg1 and IL-1β were found to have relatively high diagnostic value for CL due to L. major. Pathway analysis revealed that Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) signaling pathways are active in cutaneous lesions, providing new insights for the understanding and treatment of leishmaniasis.

中文翻译：

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通过综合生物信息学分析鉴定利什曼原虫主要感染的基因表达谱。


利什曼病小鼠模型的基因表达谱为了解病变中活跃的分子途径和发现新的诊断/治疗靶点提供了有用的信息。尽管宿主反应在预防利什曼病和促进疾病严重程度方面发挥着关键作用，但不愈合皮肤病变的机制仍然存在无法解释的方面，需要生物标志物进行靶向治疗和诊断。为了解决这个问题，通过生物信息学分析评估了感染大型利什曼原虫的 BALB/c 小鼠皮肤病变和幼稚小鼠的健康皮肤的转录谱，然后通过逆转录酶 PCR 验证结果。上调的差异表达基因中，FCGR4、CCL4、CXCL9、Arg1、IL-1β 5 个基因对 L. Major 的 CL 具有较高的诊断价值。通路分析显示，触发髓细胞表达受体 1 (TREM1) 信号通路在皮肤病变中活跃，为了解和治疗利什曼病提供了新的见解。