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Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse.
BMC Genetics Pub Date : 2020-04-28 , DOI: 10.1186/s12863-020-00843-5
Bei Li 1, 2, 3 , Xiaolong He 4 , Yiping Zhao 1, 2, 3 , Dongyi Bai 1, 2, 3 , Ming Du 1, 2, 3 , Lianjie Song 1, 2, 3 , Zhuang Liu 1, 2, 3 , Zhenchen Yin 1, 2, 3 , Dugarjaviin Manglai 1, 2, 3
Affiliation  

BACKGROUND Horse testis development and spermatogenesis are complex physiological processes. METHODS To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis. RESULTS A total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to "spermatogenesis," male gamete generation," "spermatid development" and "oocyte meiosis" were significantly involved in different stages of testis development and spermatogenesis. CONCLUSION Genes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis.
更新日期:2020-04-28
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