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Honey bee (Apis mellifera) gut microbiota promotes host endogenous detoxification capability via regulation of P450 gene expression in the digestive tract.
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-04-27 , DOI: 10.1111/1751-7915.13579 Yuqi Wu 1 , Yufei Zheng 1 , Yanan Chen 1 , Shuai Wang 1 , Yanping Chen 2 , Fuliang Hu 1 , Huoqing Zheng 1
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-04-27 , DOI: 10.1111/1751-7915.13579 Yuqi Wu 1 , Yufei Zheng 1 , Yanan Chen 1 , Shuai Wang 1 , Yanping Chen 2 , Fuliang Hu 1 , Huoqing Zheng 1
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There is growing number of studies demonstrating a close relationship between insect gut microbiota and insecticide resistance. However, the contribution of the honey bee gut microbiota to host detoxification ability has yet to be investigated. In order to address this question, we compared the expression of cytochrome P450s (P450s) genes between gut microbiota deficient (GD) workers and conventional gut community (CV) workers and compared the mortality rates and the pesticide residue levels of GD and CV workers treated with thiacloprid or tau‐fluvalinate. Our results showed that gut microbiota promotes the expression of P450 enzymes in the midgut, and the mortality rate and pesticide residue levels of GD workers are significantly higher than those of CV workers. Further comparisons between tetracycline‐treated workers and untreated workers demonstrated that antibiotic‐induced gut dysbiosis leads to attenuated expression of P450s in the midgut. The co‐treatment of antibiotics and pesticides leads to reduced survival rate and a significantly higher amount of pesticide residues in honey bees. Taken together, our results demonstrated that honey bee gut symbiont could contribute to bee health through the modification of the host xenobiotics detoxification pathways and revealed a potential negative impact of antibiotics to honey bee detoxification ability and health.
中文翻译：
蜜蜂 (Apis mellifera) 肠道微生物群通过调节消化道 P450 基因表达来促进宿主内源性解毒能力。
越来越多的研究表明昆虫肠道微生物群与杀虫剂抗性之间存在密切关系。然而，蜜蜂肠道微生物群对宿主解毒能力的贡献仍有待研究。为了解决这个问题，我们比较了肠道微生物群缺陷（GD）工人和传统肠道菌群（CV）工人之间细胞色素P450s（P450s）基因的表达，并比较了接受治疗的GD和CV工人的死亡率和农药残留水平与噻虫啉或tau氟胺氰菊酯一起使用。我们的研究结果表明，肠道菌群促进中肠P450酶的表达，GD工人的死亡率和农药残留水平显着高于CV工人。接受四环素治疗的工人和未经治疗的工人之间的进一步比较表明，抗生素引起的肠道菌群失调会导致中肠中 P450 的表达减弱。抗生素和农药的共同处理导致蜜蜂存活率降低，农药残留量显着增加。综上所述，我们的结果表明，蜜蜂肠道共生体可以通过修改宿主异生物质解毒途径来促进蜜蜂健康，并揭示抗生素对蜜蜂解毒能力和健康的潜在负面影响。
更新日期：2020-04-27
中文翻译：
蜜蜂 (Apis mellifera) 肠道微生物群通过调节消化道 P450 基因表达来促进宿主内源性解毒能力。
越来越多的研究表明昆虫肠道微生物群与杀虫剂抗性之间存在密切关系。然而，蜜蜂肠道微生物群对宿主解毒能力的贡献仍有待研究。为了解决这个问题，我们比较了肠道微生物群缺陷（GD）工人和传统肠道菌群（CV）工人之间细胞色素P450s（P450s）基因的表达，并比较了接受治疗的GD和CV工人的死亡率和农药残留水平与噻虫啉或tau氟胺氰菊酯一起使用。我们的研究结果表明，肠道菌群促进中肠P450酶的表达，GD工人的死亡率和农药残留水平显着高于CV工人。接受四环素治疗的工人和未经治疗的工人之间的进一步比较表明，抗生素引起的肠道菌群失调会导致中肠中 P450 的表达减弱。抗生素和农药的共同处理导致蜜蜂存活率降低，农药残留量显着增加。综上所述，我们的结果表明，蜜蜂肠道共生体可以通过修改宿主异生物质解毒途径来促进蜜蜂健康，并揭示抗生素对蜜蜂解毒能力和健康的潜在负面影响。
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