Humans are mostly contaminated by Salmonella through the consumption of pork- and poultry-derived food products. Therefore, a strict monitoring of Salmonella serotypes in food-producing animals is needed to limit the transmission of the pathogen to humans. Additionally, Salmonella can lead to economic loss in the food sector. Previously, a genoserotyping method using the MOL-PCR and Luminex technology was developed for the identification of the 6 Salmonella serotypes, and their variants, subjected to an official control in the Belgian food sector. In this study, 3 additional assays using the same technology were developed for the rapid and cost-effective detection of 13 dangerous highly invasive serotypes or other serotypes frequently isolated from the Belgian poultry and pork sector, i.e. Agona, Anatum, Brandenburg, Choleraesuis, Derby, Enteritidis vaccine strains, Gallinarum var. Gallinarum/Pullorum, Livingstone, Mbandaka, Minnesota, Ohio, Rissen and Senftenberg. Moreover, the previously developed first MOL-PCR assay was improved for S. Paratyphi B and serogroup O:3 detection. Finally, a Decision Support System hosted by a web application was created for an automatic and objective interpretation of the Luminex raw data. The 3 new assays and the modifications of the first assay were validated with a 100% accuracy, using 553 Salmonella and non-Salmonella strains in total.

通过食用猪肉和禽类衍生的食品，人类大多受到沙门氏菌的污染。因此，需要严格监测产食动物中沙门氏菌血清型，以限制病原体向人类的传播。此外，沙门氏菌会导致食品部门的经济损失。以前，已开发出一种利用MOL-PCR和Luminex技术进行基因分型的方法来鉴定6沙门氏菌血清型及其变种在比利时食品部门受到官方控制。在这项研究中，开发了使用相同技术的另外3种检测方法，用于快速，经济高效地检测13种危险的高侵袭性血清型或其他经常从比利时家禽和猪肉部门中分离的血清型，例如Agona，Anatum，Brandenburg，Choleraesuis，Derby ，肠炎疫苗株，Gallinarum var。Gallinarum / Pullorum，利文斯通，姆班达卡，明尼苏达州，俄亥俄州，里森和森芬特贝格。此外，先前开发的第一个MOL-PCR测定法对S进行了改进。副伤寒B和血清群O：3检测。最后，创建了一个由Web应用程序托管的决策支持系统，以自动客观地解释Luminex原始数据。总共使用了553株沙门氏菌和非沙门氏菌菌株，以100％的准确性验证了这3种新测定法和首次测定法的改进。