Abstract A facile Fourier transform infrared (FTIR) spectroscopy is developed to detect live cell activities in the transmission mode. With the use of an O ring to aid cell culture on optical windows, live cell activities can be semi-quantitatively investigated using a commercially available demountable liquid device by FTIR spectroscopy in the transmission mode. Cellular volume adaptation to osmolarity was observed by FTIR spectra for PC12 cells. The chemical information upon growth of human cervical cancer cell line, HeLa cells, were investigated by FTIR at interval incubation time. Glycogen enrichment concurring with cell proliferation was illustrated, but the dissipation of glycogen upon subculturing is to be understood. Further comparison of the CaSki cells with its counterpart Ect1/E6E7 cells revealed bands features for the discrimination of the two cell types. These results demonstrate that the developed protocol can be complementary to microincubators and facilitate chemical analyses of live cellular activities by FTIR spectroscopy in the transmission mode.

中文翻译：

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通过傅里叶变换红外 (FTIR) 光谱在透射模式下对活细胞活性进行化学分析

摘要 开发了一种简便的傅里叶变换红外 (FTIR) 光谱，以在透射模式下检测活细胞活动。通过使用 O 形环来帮助在光学窗口上进行细胞培养，可以使用市售的可拆卸液体装置通过 FTIR 光谱在透射模式下对活细胞活动进行半定量研究。通过 PC12 细胞的 FTIR 光谱观察到细胞体积对渗透压的适应性。通过 FTIR 在间隔孵育时间研究人宫颈癌细胞系 HeLa 细胞生长的化学信息。说明了与细胞增殖同时发生的糖原富集，但要了解继代培养后糖原的消散。CaSki 细胞与其对应的 Ect1/E6E7 细胞的进一步比较揭示了区分两种细胞类型的条带特征。这些结果表明, 开发的协议可以作为微型孵化器的补充, 并有助于通过 FTIR 光谱在传输模式下对活细胞活动进行化学分析。