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Investigation of the survival viability of cervical cancer cells (HeLa) under visible light induced photo-catalysis with facile synthesized WO3/ZnO nanocomposite.
Saudi Journal of Biological Sciences Pub Date : 2020-04-27 , DOI: 10.1016/j.sjbs.2020.04.038
Rasha A AbuMousa 1 , Umair Baig 2 , Mohammed A Gondal 3 , Mohamed A Dastageer 3 , Mohamad S AlSalhi 4 , Belal Moftah 5, 6 , Fulwah Yahya Alqahtani 7 , Sultan Akhter 8 , Fadilah Sfouq Aleanizy 6
Affiliation  

The photo catalytic degradation, a proven chemical process used for the decontamination of organic/inorganic pollutants and microorganisms in water was implemented. In this work for the selective killing of cervical cancer cells (HeLa cells) by using nano-composite of ZnO (Zinc Oxcide), WO3 (tungsten oxide) and (n-WO3/ZnO) as a photo-catalyst under the irradiation of visible light. All the three nanostructured semiconducting materials (WO3, ZnO and n-WO3/ZnO) were synthesized by facile chemical precipitation method and their morphological and optical characterization studies were carried out to elucidate the observed enhancement in the photo-catalytic killing of HeLa cancer cells with n-WO3/ZnO as a photo-catalyst. After 60 min of photo-catalytic reaction with n-WO3/ZnO as a photo-catalyst, a survival viability of HeLa cancer cells as low as 15% was achieved (nearly 85% of killing), as compared to 65% of HeLa cancer cell survival viability (nearly 35% of killing) with individual use of WO3 and ZnO as photo-catalysts under the same irradiation and experimental conditions. This improved photo-catalytic killing of HeLa cancer cells using n-WO3/ZnO in the visible spectral region is attributed to the enhanced visible light absorption and reduced electron hole recombination, characteristically brought about in the n-WO3/ZnO composite material. As photo-catalytic killing of the cancer cells can be selective, localized and reasonably efficient, in principle, this method can be considered as a non-invasive targeted treatment option for killing any type of cancer cells. HeLa cells, in particular are the cervical cancer cell and the tumors in and around cervix, containing HeLa cells can be non-surgically accessed and photo-catalytically treated with appropriate photo-catalyst and light source.



中文翻译:

用方便的合成WO3 / ZnO纳米复合材料在可见光诱导的光催化下研究宫颈癌细胞(HeLa)的生存能力。

实施了光催化降解,这是一种用于水中有机/无机污染物和微生物净化的成熟化学方法。在这项工作中,通过使用ZnO氧化锌),WO 3(氧化钨)和(n-WO 3 / ZnO)的纳米复合材料选择性杀死宫颈癌细胞(HeLa细胞)作为可见光照射下的光催化剂。所有三种纳米结构的半导体材料(WO3,ZnO和n-WO3 / ZnO)都是通过简便的化学沉淀法合成的,并对其形态和光学特性进行了研究,以阐明观察到的增强作用对HeLa癌细胞的光催化杀伤作用。 n-WO3 / ZnO作为光催化剂。在使用n-WO 3 / ZnO作为光催化剂进行60分钟的光催化反应后,HeLa癌细胞的生存能力可低至15%(杀死率将近85%),而HeLa癌细胞的存活率仅为65%单独使用WO 3ZnO的癌细胞存活力(几乎杀死细胞的35%)在相同的辐射和实验条件下作为光催化剂。在可见光谱区域中使用n-WO 3 / ZnO改善HeLa癌细胞的光催化杀伤力归因于增强的可见光吸收和减少的电子空穴复合,这是n-WO 3 / ZnO复合材料所特有的。原则上,由于光催化杀死癌细胞可以是选择性的,局部的且相当有效的,因此该方法可以被视为杀死任何类型癌细胞的非侵入性靶向治疗选择。HeLa细胞,特别是子宫颈癌细胞和子宫颈内和周围的肿瘤,含有可以非手术方式访问HeLa细胞,并使用适当的光催化剂和光源进行光催化处理。

更新日期:2020-04-27
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