Biological soil crusts (BSC) (Cyanobacteria) play an important role in the soil nitrogen fixation and soil stabilization. However, limited researches were carried out about the diversity and distribution of Bio crust in sacred forests. The study aims to identify the distribution of cyanobacteria in biological soil crusts from different sacred groves of Ariyalur and Pudukottai, Tamil Nadu. We identified following microbes of Microcoleus, Scytonema, Anabaena and Nostoc in biological soil crust. A surface experiment was conducted for the efficacy of biological soil crusts on crops seedling growths. The efficacy was assessed by measuring the root & shoot length, dry & fresh weight of the plant, total chlorophyll, protein & carbohydrate content, with reducing sugar. The plant growth was higher in biocrust inoculated pot than the control. In corresponding nitrogenase activity was determined by the acetylene reduction assay, and phytohormones documentation was executed by the high-performance liquid chromatography. The results indicating that the biological soil crusts are the promising factors influencing the plant growth by plant growth-stimulating auxins and nitrogenase activity.

生物土壤结皮（BSC）（蓝细菌）在土壤固氮和土壤稳定方面发挥着重要作用。然而，对于神圣森林中生物结皮的多样性和分布进行的研究有限。该研究旨在确定泰米尔纳德邦 Ariyalur 和 Pudukottai 不同神圣树林中生物土壤结皮中蓝藻的分布。我们在生物土壤结皮中鉴定出以下微生物：微鞘藻、藻丝藻、鱼腥藻和发菜。进行了表面实验，研究生物土壤结皮对农作物幼苗生长的功效。通过测量根和芽的长度、植物的干重和鲜重、总叶绿素、蛋白质和碳水化合物含量以及还原糖来评估功效。接种生物结皮的盆中的植物生长高于对照。通过乙炔还原测定测定相应的固氮酶活性，并通过高效液相色谱法进行植物激素记录。结果表明，生物土壤结皮是通过刺激植物生长的生长素和固氮酶活性影响植物生长的有希望的因素。