A chiral binuclear Ni(II) complex [Ni 2 ( o -van-ala) 2 (phen) 2 ] ( o -van-ala = a Schiff base derived from o -vanillin and l -alanine, phen = 1,10-phenanthroline) has been synthesized and characterized by physicochemical and spectroscopic methods. Single-crystal X-ray diffraction result showed that the Ni(II) complex contains two nickel centers, which are further bridged and coordinated by two o -van-ala Schiff base and two phen ligands in a cis -conformation. Each nickel atom is six-coordinated in a distorted NiN 3 O 3 octahedral geometry. The interaction of the Ni(II) complex with calf thymus DNA (CT-DNA) has been investigated by spectroscopic methods. Based on results obtained, an intercalative binding mode has been proposed. In addition, the interaction between the Ni(II) complex and bovine serum albumin (BSA) has also been studied by various spectroscopic techniques. Results indicated that the Ni(II) complex can quench the intrinsic fluorescence of BSA in a static quenching process. The binding constant ( K b ) and the number of binding sites ( n ) were calculated to be 2.92 × 10 6 M −1 and 1.21, respectively. Site-selective competitive binding experiment indicated that the binding site of the Ni(II) complex locates in site I in sub-domains IIA of BSA. The Ni(II) complex also displayed a significant superoxide anion scavenging activity.

中文翻译：

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具有席夫碱配体的手性双核镍 (II) 复合物：合成、晶体结构、DNA/BSA 结合相互作用和 SOD 活性

手性双核 Ni(II) 配合物 [Ni 2 ( o -van-ala) 2 (phen) 2 ] ( o -van-ala = 衍生自邻香兰素和 l -丙氨酸的希夫碱，phen = 1,10-菲咯啉）已通过物理化学和光谱方法合成和表征。单晶 X 射线衍射结果表明，Ni(II) 配合物包含两个镍中心，这些中心由两个 o-van-ala Schiff 碱和两个顺式构象的 phen 配体进一步桥接和配位。每个镍原子在扭曲的 NiN 3 O 3 八面体几何结构中是六配位的。Ni(II) 复合物与小牛胸腺 DNA (CT-DNA) 的相互作用已通过光谱方法进行了研究。根据获得的结果，提出了一种插入式结合模式。此外，Ni(II) 络合物与牛血清白蛋白 (BSA) 之间的相互作用也已通过各种光谱技术进行了研究。结果表明，Ni(II)配合物可以在静态猝灭过程中猝灭BSA的固有荧光。结合常数（K b ）和结合位点的数量（ n ）经计算分别为2.92 × 10 6 M -1 和1.21。位点选择性竞争结合实验表明，Ni(II)复合物的结合位点位于BSA IIA子域中的位点I。Ni(II) 配合物还显示出显着的超氧阴离子清除活性。分别为 92 × 10 6 M -1 和 1.21。位点选择性竞争结合实验表明，Ni(II)复合物的结合位点位于BSA IIA子域中的位点I。Ni(II) 配合物还显示出显着的超氧阴离子清除活性。分别为 92 × 10 6 M -1 和 1.21。位点选择性竞争结合实验表明，Ni(II)复合物的结合位点位于BSA IIA子域中的位点I。Ni(II) 配合物还显示出显着的超氧阴离子清除活性。