Shallot (Allium cepa var. aggregatum), a small bulb onion, is widely grown in the world. We previously reported a droplet-vitrification for cryopreservation of in vitro-grown shoot tips of shallot genotype ‘10603’. The present study further evaluated rooting, vegetative growth, bulb production and contents of biochemical compounds, as well as genetic stability in cryo-derived plants. The results showed no significant differences in rooting, vegetative growth, bulb production and contents of soluble sugars and flavonols between the cryo- and in vitro-derived plants. Analyses of ISSR and AFLP markers did not detect any polymorphic bands in the cryo-derived plants. These results indicate rooting and vegetative growth ability, biochemical compounds and genetic stability were maintained in cryo-derived plants. The present study provides experimental evidences that support the use of cryopreservation method for long-term preservation of genetic resources of shallots and other Allium species.

葱（洋葱变种aggregatum），一种小鳞茎洋葱，在世界范围内广泛种植。我们先前曾报道过一种液滴玻璃化法，用于冷冻保存葱基因型“ 10603”的体外生长梢。本研究进一步评估了生根，营养生长，鳞茎产量和生化化合物的含量以及低温衍生植物的遗传稳定性。结果表明，低温和离体植物在生根，营养生长，鳞茎产量以及可溶性糖和黄酮醇含量上均无显着差异。ISSR和AFLP标记的分析未检测到冷冻来源的植物中的任何多态性条带。这些结果表明在冷冻来源的植物中保持了生根和营养生长能力，生化化合物和遗传稳定性。葱属种。