In conventional preparation of enzyme embedded organic-inorganic hybrid flower-like nanostructures, usually it requires three days which is time-consuming and limits their widespread applications. In this context, alpha-amylase hybrid bio-microflowers were prepared by simple, efficient and rapid method in the presence of chitosan via inotropic gelation and biomineralization approach. The hybrid bio-microflowers was synthesized within 6 h with ∼140 % enhanced catalytic activity. The prepared hybrid bio-microflowers were characterized by FT-IR, SEM, and PXRD. The hybrid bio-microflowers exhibited higher rate of reaction (Vmax) and outstanding thermo-stability (in the temperature range 55-75 °C). Further, hybrid bio-microflowers showed magnificent reusability (upto eight cycles) and long-term storage stability (for about 30 days). In the end, in starch hydrolytic study, immobilized alpha-amylase exhibited higher hydrolytic potential towards corn, wheat and potato starch as compared to free form. This designed hybrid bio-microflowers can be employed as an engineered biocatalyst in industrial applications.

中文翻译：

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具有增强活性的α-淀粉酶快速自组装杂交生物微花。

在常规的酶包埋的有机-无机杂化花状纳米结构的常规制备中，通常需要三天，这很耗时并且限制了它们的广泛应用。在这种情况下，在壳聚糖存在下，通过正性凝胶化和生物矿化方法，通过简单，有效和快速的方法制备了α-淀粉酶杂化生物微花。杂种生物微花在6小时内合成，催化活性提高了约140％。通过FT-IR，SEM和PXRD对制备的杂种生物微花进行了表征。杂种生物微花显示出更高的反应速率（Vmax）和出色的热稳定性（在55-75°C的温度范围内）。此外，杂种生物微花显示出极好的可重复使用性（最多八个循环）和长期存储稳定性（约30天）。到底，在淀粉水解研究中，与游离形式相比，固定化的α-淀粉酶对玉米，小麦和马铃薯淀粉具有更高的水解潜力。这种设计的杂化生物微花可以在工业应用中用作工程化的生物催化剂。