Overexpression of blue light receptor AaCRY1 improves artemisinin content in Artemisia annua L. ,Biotechnology and Applied Biochemistry

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Overexpression of blue light receptor AaCRY1 improves artemisinin content in Artemisia annua L.
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-04-27 , DOI: 10.1002/bab.1931
Xueqing Fu ₁ , Yilong He ₁ , Ling Li ₁ , Limei Zhao ₂ , Yuting Wang ₁ , Hongmei Qian ₁ , Xiaofen Sun ₁ , Kexuan Tang ₁ , Jingya Zhao ₁

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Artemisinin, an effective antimalarial compound, is isolated from the medicinal plant Artemisia annua L. However, because of the low content of artemisinin in A. annua, the demand of artemisinin exceeds supply. Previous studies show that the artemisinin biosynthesis is promoted by light in A. annua. Cryptochrome1 (CRY1) is involved in many processes in the light response. In this study, AaCRY1 was cloned from A. annua. Overexpressing AaCRY1 in Arabidopsis thaliana cry1 mutant resulted in blue‐light‐dependent short hypocotyl phenotype and short coleoptile under blue light. Yeast two‐hybrid and subcellular colocalization showed that AaCRY1 interacted with AtCOP1 (ubiquitin E3 ligase CONSTITUTIVE PHOTOMORPHOGENIC1). Overexpression of AaCRY1 in transgenic A. annua increased the artemisinin content. When AaCRY1 was overexpressed in A. annua driven by the CYP71AV1 (cytochrome P450 dependent amorpha‐4,11‐diene 12‐hydroxylase) promoter, the artemisinin content was 1.6 times higher than that of the control. Furthermore, we expressed the C terminal of AaCRY1(CCT) involved a GUS‐CCT fusion protein in A. annua. The results showed that the artemisinin content was increased to 1.7‐ to 2.4‐fold in GUS‐CCT transgenic A. annua plants. These results demonstrate that overexpression of GUS‐CCT is an effective strategy to increase artemisinin production in A. annua.

中文翻译：

蓝光受体AaCRY1的过表达提高了青蒿中青蒿素的含量。

青蒿素是一种有效的抗疟化合物，是从药用植物青蒿中分离出来的。但是，由于青蒿中青蒿素含量低，青蒿素的需求超过了供应。以往的研究表明，青蒿素生物合成受光促进青蒿。Cryptochrome1（CRY1）参与光响应的许多过程。在这项研究中，从A. annua克隆了AaCRY1。在拟南芥cry1中过表达AaCRY1突变导致蓝光依赖性短胚轴表型和短胚芽鞘在蓝光下。酵母双杂交和亚细胞共定位表明，AaCRY1与AtCOP1（泛素E3连接酶组成型光生化蛋白1）相互作用。AaCRY1在转基因农杆菌中的过表达增加了青蒿素的含量。当AaCRY1在过表达青蒿由从动CYP71AV1（细胞色素P450依赖性紫穗槐-4,11-二烯12-羟化酶）启动子，青蒿素含量比对照高1.6倍。此外，我们表达了AaCRY1（CCT）的C末端参与了A. annua中的GUS-CCT融合蛋白。。结果表明，在GUS-CCT转基因青蒿植物中，青蒿素含量增加到1.7到2.4倍。这些结果表明的过度表达GUS-CCT是增加青蒿素产量的有效策略青蒿。

更新日期：2020-04-27

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