The present work isolated a CBF/DREB1 gene from mangrove Bruguiera gymnorrhiza (BgCBF1) and compared its expression levels in various tissues under normal condition and cold stress, and in leaves exposed to various environmental stimuli. Results showed that the BgCBF1 deduced protein showed almost 100% similarities to that of AcCBF1 from Aegiceras corniculatum and AmCBF1 from Avicennia marina. Real-time quantitative PCR analysis showed that BgCBF1 gene displayed constitute expression in leaf, stem and root samples of plantlets under normal condition, but with different expression levels and tissue preference. When exposed to cold, BgCBF1 could be rapidly, slightly and transiently induced in all tissues. Furthermore, the BgCBF1 gene in leaves displayed a transient and small induction after salt and drought (PEG) exposure, while exhibited relatively high up-regulated expression after the phytohormone abscisic acid (ABA) treatment. These results suggest that the BgCBF1 gene may participate in the ABA mediated development and protection of plant against cold and drought. Further studies on its promoters and downstream genes will be needed to better understand its functions.

中文翻译：

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红树林Bruguiera Gymnorrhiza中CBF转录因子基因的分离和表达分析。

目前的工作是从红树林布鲁吉亚草（Bruguiera Gymnorrhiza）（BgCBF1）中分离出CBF / DREB1基因，并比较了其在正常条件下和冷胁迫下在各种组织中以及在各种环境刺激下的叶片中的表达水平。结果表明，BgCBF1推导的蛋白质与来自Aegiceras corniculatum的AcCBF1和来自Avicennia marina的AmCBF1几乎显示100％相似性。实时定量PCR分析表明，所显示的BgCBF1基因在正常条件下在幼苗的叶片，茎和根样品中表达，但表达水平和组织偏好不同。当暴露于寒冷中时，BgCBF1可以在所有组织中快速，轻微和短暂地诱导。此外，盐和干旱（PEG）暴露后，叶片中的BgCBF1基因显示出短暂而短暂的诱导，而在植物激素脱落酸（ABA）处理后表现出较高的上调表达。这些结果表明，BgCBF1基因可能参与ABA介导的植物的发育和对寒冷和干旱的保护。为了更好地了解其功能，需要对其启动子和下游基因进行进一步研究。