Abstract

The effect of stilbene polyphenols (resveratrol and pinosilvin) and flavonoids (dihydromyricetin, epigallocatechin, and dihydroquercetin) on producing the reactive oxygen species (ROS) due to cell respiration using the isolated mitochondria of the fungus Dipodascus (Endomyces) magnusii was studied. It was shown that the inhibition of the ROS generation with stilbenes is related to both the oxygen consumption inhibition and their antioxidant properties. The degree of manifestation of polyphenol antioxidant properties depended on the number of hydroxyl groups in a polyphenol molecule. Of the flavonoids tested, dihydromyricetin was most active in reducing the ROS generation, but its inhibitory effect on oxygen consumption by mitochondria was significantly lower as compared to that of the stilbenes. The data obtained show that the mechanism of reducing the ROS generation with polyphenols is associated with both the respiratory inhibition and their antioxidant properties.

中文翻译：

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天然多酚对分离的酵母双歧线粒体线粒体的影响。

摘要

由于分离出的线粒体真菌线粒体（线虫）的线粒体，由于细胞的呼吸作用，二苯乙烯多酚（白藜芦醇和Pinosilvin）和类黄酮（二氢杨梅素，表没食子儿茶素和二氢槲皮素）对产生活性氧（ROS）的影响被研究了。结果表明，对苯二酚对ROS生成的抑制与氧消耗抑制及其抗氧化性能有关。多酚抗氧化剂性能的表现程度取决于多酚分子中的羟基数。在所测试的类黄酮中，二氢杨梅素在减少ROS生成方面最活跃，但与对苯二酚相比，其对线粒体耗氧量的抑制作用明显较低。获得的数据表明，多酚减少ROS生成的机制与呼吸抑制作用及其抗氧化特性有关。