Abstract The effect of the yeast endopolyphosphatase Ppn2 overproduction on the metabolism of inorganic polyphosphates in Saccharomyces cerevisiae yeast was studied. Expression of the PPN2 gene under control of the strong constitutive promoter of glyceraldehyde 3-phosphate dehydrogenase gene ( PKG1 ) led to a significant increase in the endopolyphosphatase activity stimulated by cobalt/zinc ions. This activity was present in both soluble and membrane subcellular fractions; it was higher toward long-chain polyphosphates and could be stimulated by ADP. The content of short-chain polyphosphates in the cells of the overexpressing strain was ~2.5 times higher compared to the parent strain. The cells overexpressing Ppn2 were more resistant to peroxide and alkali. The role of short-chain polyphosphates in the adaptation to these stress factors is discussed.

中文翻译：

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过表达 Ppn2 的酿酒酵母酵母的无机多磷酸盐和生理特性

摘要 研究了酵母内多磷酸酶 Ppn2 过量产生对酿酒酵母中无机多磷酸盐代谢的影响。PPN2 基因在 3-磷酸甘油醛脱氢酶基因 (PKG1) 的强组成型启动子控制下的表达导致钴/锌离子刺激的内聚磷酸酶活性显着增加。这种活性存在于可溶性和膜亚细胞组分中；它对长链聚磷酸盐更高，可以被 ADP 刺激。与亲本菌株相比，过表达菌株细胞中短链聚磷酸盐的含量高约 2.5 倍。过表达 Ppn2 的细胞对过氧化物和碱的抵抗力更强。