We noticed that syphilis patients seem to be more susceptible to diabetes and the lesions often involve the kidneys, but the pathogenesis is not yet completely understood. In this study, microarray analysis was performed to investigate the dysregulated expressed genes (DEGs) in rabbit model of syphilis combined with diabetes. A total of 1045 genes were identified to be significantly differentially expressed, among which 571 were up-regulated and 474 were down-regulated (≥ 2.0fold, p < 0.05). Using the database visualization and integration discovery for the Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analysis. The downregulated DEGs were significantly enriched for biosynthesis of antibiotics, carbon metabolism and protein digestion, while the upregulated DEGs were mainly enriched for cancer and PI3K-Akt signaling pathway. Molecular Complex Detection (MCODE) plugins were used to visualize protein-protein interaction (PPI) network of DEGs and Screening for hub genes and gene modules. ALB, FN1, CASP3, MMP9, IL8, CTGF, STAT3, IGF1, VCAM-1 and HGF were filtrated as the hub genes according to the degree of connectivity from the PPI network. To the best of our knowledge, this study is the first to comprehensively identify the expression patterns of dysregulated genes in syphilis combined with diabetes, providing a basis for revealing the underlying pathogenesis of syphilis combined with diabetes and exploring the goals of therapeutic intervention.

中文翻译：

---

梅毒合并糖尿病的关键基因和途径的鉴定：一项生物信息学研究。

我们注意到梅毒患者似乎更容易患糖尿病，并且病变常累及肾脏，但发病机理尚未完全明了。在这项研究中，进行了微阵列分析以研究梅毒合并糖尿病的兔子模型中表达失调的基因（DEGs）。总共鉴定出1045个基因显着差异表达，其中571个上调而474个下调（≥2.0倍，p <0.05）。使用数据库可视化和整合发现进行京都基因和基因组百科全书（KEGG）途径富集分析。下调的DEGs大量富集于抗生素的生物合成，碳代谢和蛋白质消化，而上调的DEGs主要富集于癌症和PI3K-Akt信号通路。分子复合物检测（MCODE）插件用于可视化DEG的蛋白质-蛋白质相互作用（PPI）网络，并筛选中心基因和基因模块。根据来自PPI网络的连接程度，将ALB，FN1，CASP3，MMP9，IL8，CTGF，STAT3，IGF1，VCAM-1和HGF过滤为中枢基因。据我们所知，这项研究是首次全面鉴定梅毒合并糖尿病中失调基因的表达模式，为揭示梅毒合并糖尿病的潜在发病机制和探索治疗干预目标提供了基础。根据来自PPI网络的连接程度，将VCAM-1和HGF过滤为中枢基因。据我们所知，这项研究是首次全面鉴定梅毒合并糖尿病中失调基因的表达模式，为揭示梅毒合并糖尿病的潜在发病机制和探索治疗干预目标提供了基础。根据来自PPI网络的连接程度，将VCAM-1和HGF过滤为中枢基因。据我们所知，这项研究是首次全面鉴定梅毒合并糖尿病中失调基因的表达模式，为揭示梅毒合并糖尿病的潜在发病机制和探索治疗干预目标提供了基础。