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Influence of 3-nitropropionic acid on physiological and behavioral responses in zebrafish larvae and adults.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-04-27 , DOI: 10.1016/j.cbpc.2020.108772 Melissa Talita Wiprich 1 , Rodrigo Zanandrea 1 , Stefani Altenhofen 2 , Carla Denise Bonan 2
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-04-27 , DOI: 10.1016/j.cbpc.2020.108772 Melissa Talita Wiprich 1 , Rodrigo Zanandrea 1 , Stefani Altenhofen 2 , Carla Denise Bonan 2
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Long-term treatment with 3-nitropropionic acid (3-NPA), a toxin derived from plants and fungi, may reproduce symptoms and biochemical characteristics of Huntington's disease (HD). Our study evaluated the effects of 3-NPA on the physiological and behavioral responses in zebrafish larvae and adults. Larvae exposed to 0.1, 0.2, or 0.5 mM 3-NPA exhibited an increase in heart rate at 2- and 5-days post-fertilization (dpf). There was a decrease in the ocular distance at 5 dpf with 0.05 mM 3-NPA treatment. However, 3-NPA did not alter larval locomotor parameters. Adult zebrafish received 3-NPA intraperitoneal injections (a total of seven injections at doses 10, 20, or 60 mg/kg every 96 h) and showed a decrease in body weight , locomotion and aggressive behavior. No changes were observed in anxiety-like behavior and social interaction between 3-NPA-exposed animals and control groups. However, 3-NPA-treated animals (at 60 mg/kg) demonstrated impaired long-term aversive memory. Overall, 3-NPA exposure induced morphological and heart rate alterations in zebrafish larvae. Additionally, our study showed behavioral changes in zebrafish that were submitted to long-term 3-NPA treatment, which could be related to HD symptoms.
中文翻译：
3-硝基丙酸对斑马鱼幼虫和成虫的生理和行为反应的影响。
使用3-硝基丙酸（3-NPA）（一种源自植物和真菌的毒素）进行长期治疗可能会重现亨廷顿舞蹈病（HD）的症状和生化特征。我们的研究评估了3-NPA对斑马鱼幼虫和成虫的生理和行为反应的影响。暴露于0.1、0.2或0.5 mM 3-NPA的幼虫在受精后2天和5天（dpf）表现出心率增加。用0.05 mM 3-NPA治疗在5 dpf时的眼距降低。但是，3-NPA不会改变幼虫的运动参数。成年斑马鱼接受3-NPA腹膜内注射（每96小时共7次注射，剂量分别为10、20或60 mg / kg），体重，运动和攻击行为均有所降低。在暴露于3-NPA的动物与对照组之间的焦虑样行为和社交互动中未发现任何变化。但是，经3-NPA处理的动物（60 mg / kg）表现出长期厌恶记忆受损。总体而言，暴露于3-NPA会引起斑马鱼幼虫的形态和心率变化。此外，我们的研究表明，长期接受3-NPA治疗的斑马鱼的行为变化可能与HD症状有关。
更新日期：2020-04-27
中文翻译：
3-硝基丙酸对斑马鱼幼虫和成虫的生理和行为反应的影响。
使用3-硝基丙酸（3-NPA）（一种源自植物和真菌的毒素）进行长期治疗可能会重现亨廷顿舞蹈病（HD）的症状和生化特征。我们的研究评估了3-NPA对斑马鱼幼虫和成虫的生理和行为反应的影响。暴露于0.1、0.2或0.5 mM 3-NPA的幼虫在受精后2天和5天（dpf）表现出心率增加。用0.05 mM 3-NPA治疗在5 dpf时的眼距降低。但是，3-NPA不会改变幼虫的运动参数。成年斑马鱼接受3-NPA腹膜内注射（每96小时共7次注射，剂量分别为10、20或60 mg / kg），体重，运动和攻击行为均有所降低。在暴露于3-NPA的动物与对照组之间的焦虑样行为和社交互动中未发现任何变化。但是，经3-NPA处理的动物（60 mg / kg）表现出长期厌恶记忆受损。总体而言，暴露于3-NPA会引起斑马鱼幼虫的形态和心率变化。此外，我们的研究表明，长期接受3-NPA治疗的斑马鱼的行为变化可能与HD症状有关。
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